Visioli F, Caruso D, Plasmati E, Patelli R, Mulinacci N, Romani A, Galli G, Galli C
Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milan.
Free Radic Res. 2001 Mar;34(3):301-5. doi: 10.1080/10715760100300271.
Hydroxytyrosol is the most potent phenolic antioxidant of olive oil and olive mill waste water (OMWW) and its biological activities have stimulated research on its potential role in cardiovascular protection. However, evidence of the absorption of OMWW phenolics and on their possible in vivo activity has, until now, never been provided. Three groups male Sprague-Dawley rats were administered 1, 5, or 10 mg/Kg of the OMWW extract, respectively, providing 41.4, 207, and 414 microg/Kg of hydroxytyrosol, respectively. Urine was collected for 24 h and the urinary levels of hydroxytyrosol were quantified by mass spectrometry. Hydroxytyrosol was dose-dependently (R(2) = 0.95) absorbed and excreted in the urines mostly as a glucuronide conjugate. Further, the administration of an hydroxytyrosol-rich OMWW extract (10 mg/kg) to the rats was also associated with an increase of their plasma antioxidant capacity. Future experiments will eventually further clarify its metabolic fate and its in vivo actions.
羟基酪醇是橄榄油和橄榄果渣废水（OMWW）中最有效的酚类抗氧化剂，其生物活性激发了人们对其在心血管保护中潜在作用的研究。然而，迄今为止，从未有过关于OMWW酚类物质吸收及其体内可能活性的证据。将三组雄性斯普拉格-道利大鼠分别给予1、5或10mg/kg的OMWW提取物，分别提供41.4、207和414μg/kg的羟基酪醇。收集24小时尿液，通过质谱法定量尿液中羟基酪醇的水平。羟基酪醇呈剂量依赖性吸收（R² = 0.95），并主要以葡萄糖醛酸共轭物的形式排泄到尿液中。此外，给大鼠施用富含羟基酪醇的OMWW提取物（10mg/kg）也与其血浆抗氧化能力的增加有关。未来的实验最终将进一步阐明其代谢命运及其体内作用。
