评估一种用于检测从脑脊液标本中扩增出的肠道病毒逆转录聚合酶链反应产物的商业DNA酶免疫测定法。
Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.
作者信息
Young P P, Buller R S, Storch G A
机构信息
The Departments of Pathology, Washington University School of Medicine at St. Louis Children's Hospital, St. Louis, Missouri 63110, USA.
出版信息
J Clin Microbiol. 2000 Nov;38(11):4260-1. doi: 10.1128/JCM.38.11.4260-4261.2000.
Abstract
We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.
摘要
我们评估了DiaSorin DNA酶免疫分析（DEIA）试剂盒用于检测从脑脊液中扩增出的肠道病毒逆转录聚合酶链反应（RT-PCR）产物。以0.05的光密度作为吸光度临界值，198份标本中有35%经PCR检测为阳性，而培养阳性率为16%。DEIA快速且灵敏，有助于临床实验室开展肠道病毒RT-PCR检测。
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