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Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.评估一种用于检测从脑脊液标本中扩增出的肠道病毒逆转录聚合酶链反应产物的商业DNA酶免疫测定法。
J Clin Microbiol. 2000 Nov;38(11):4260-1. doi: 10.1128/JCM.38.11.4260-4261.2000.
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Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid.应用带有内部阳性对照的实时聚合酶链反应检测和定量脑脊液中的肠道病毒。
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引用本文的文献

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Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection.通过离心增强抗原检测法检测临床标本中肠道病毒的细胞病变前效应。
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本文引用的文献

1
Multicenter quality assessment of PCR methods for detection of enteroviruses.用于检测肠道病毒的聚合酶链反应（PCR）方法的多中心质量评估
J Clin Microbiol. 1999 May;37(5):1409-14. doi: 10.1128/JCM.37.5.1409-1414.1999.
2
Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens.评估一种市售逆转录-聚合酶链反应检测法在诊断存档及前瞻性收集的脑脊液标本中肠道病毒感染的应用。
J Clin Microbiol. 1998 Jun;36(6):1741-5. doi: 10.1128/JCM.36.6.1741-1745.1998.
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Reproducibility of AMPLICOR enterovirus PCR test results.AMPLICOR肠道病毒聚合酶链反应检测结果的可重复性
J Clin Microbiol. 1997 Dec;35(12):3301-2. doi: 10.1128/jcm.35.12.3301-3302.1997.
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Clinical utility of the polymerase chain reaction for diagnosis of enteroviral meningitis in infancy.聚合酶链反应在婴儿肠道病毒性脑膜炎诊断中的临床应用
J Pediatr. 1997 Sep;131(3):393-7. doi: 10.1016/s0022-3476(97)80064-9.
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Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.通过磁珠提取和聚合酶链反应检测临床标本中的肠道病毒RNA对肠道病毒感染进行快速诊断。
J Clin Microbiol. 1993 Jan;31(1):31-8. doi: 10.1128/jcm.31.1.31-38.1993.
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Enteroviral meningitis in infancy: potential role for polymerase chain reaction in patient management.婴儿肠道病毒性脑膜炎：聚合酶链反应在患者管理中的潜在作用。
Pediatrics. 1994 Aug;94(2 Pt 1):157-62.
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Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.采用比色微孔检测法的聚合酶链反应诊断肠道病毒脑膜炎。
J Clin Microbiol. 1994 Oct;32(10):2590-2. doi: 10.1128/jcm.32.10.2590-2592.1994.
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Detection of enteroviruses by spot hybridization.斑点杂交法检测肠道病毒
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Enzymatic RNA amplification of the enteroviruses.肠道病毒的酶促RNA扩增
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评估一种用于检测从脑脊液标本中扩增出的肠道病毒逆转录聚合酶链反应产物的商业DNA酶免疫测定法。

Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.

作者信息

Young P P, Buller R S, Storch G A

机构信息

The Departments of Pathology, Washington University School of Medicine at St. Louis Children's Hospital, St. Louis, Missouri 63110, USA.

出版信息

J Clin Microbiol. 2000 Nov;38(11):4260-1. doi: 10.1128/JCM.38.11.4260-4261.2000.

DOI:10.1128/JCM.38.11.4260-4261.2000
PMID:11060104
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87577/
Abstract

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

摘要

我们评估了DiaSorin DNA酶免疫分析（DEIA）试剂盒用于检测从脑脊液中扩增出的肠道病毒逆转录聚合酶链反应（RT-PCR）产物。以0.05的光密度作为吸光度临界值，198份标本中有35%经PCR检测为阳性，而培养阳性率为16%。DEIA快速且灵敏，有助于临床实验室开展肠道病毒RT-PCR检测。