AMPLICOR肠道病毒聚合酶链反应检测结果的可重复性
Reproducibility of AMPLICOR enterovirus PCR test results.
作者信息
Rotbart H A
机构信息
University of Colorado Health Sciences Center, Denver 80262, USA.
出版信息
J Clin Microbiol. 1997 Dec;35(12):3301-2. doi: 10.1128/jcm.35.12.3301-3302.1997.
Abstract
The reproducibility of AMPLICOR enterovirus PCR test results was determined with clinical samples of cerebrospinal fluid, serum, urine, and throat and rectal swabs. Among 608 samples from which duplicate aliquots were run simultaneously, only seven pairs gave discordant results. Among 104 samples from which duplicate aliquots were run in separate assays, no discordance was seen. Overall, the reproducibility of test kit results was 99% (705 of 712).
摘要
利用脑脊液、血清、尿液以及咽喉和直肠拭子的临床样本，测定了AMPLICOR肠道病毒聚合酶链反应（PCR）检测结果的可重复性。在同时进行重复等分试样检测的608份样本中，只有7对结果不一致。在分别进行重复等分试样检测的104份样本中，未发现不一致情况。总体而言，试剂盒结果的可重复性为99%（712份样本中的705份）。
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