Leal-Klevezas D S, Martínez-Vázquez I O, Cuevas-Hernández B, Martínez-Soriano J P
Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico.
Clin Diagn Lab Immunol. 2000 Nov;7(6):945-6. doi: 10.1128/CDLI.7.6.945-946.2000.
Preserving blood samples for shipping and later DNA extraction has been performed by cooling, freezing, drying, freeze-drying, and protease treatment, among other methods. Most methods to preserve field samples for further DNA extraction do not prevent cellular and DNA damage or are useful only in preserving them for short periods. This report introduces a novel method for blood and tissue that allows preservation in freezing temperatures for a prolonged period of time. The solution reported here (20% ethylene glycol-propylene glycol) preserves cells and tissues integrity, as judged by microscopic analysis, and improves DNA yield and quality.
为了运输和后续的DNA提取而保存血样的方法有冷却、冷冻、干燥、冻干和蛋白酶处理等。大多数用于保存野外样本以便进一步进行DNA提取的方法并不能防止细胞和DNA损伤，或者仅在短时间内保存样本时有用。本报告介绍了一种针对血液和组织的新方法，该方法可在冷冻温度下长时间保存样本。通过显微镜分析判断，此处报道的溶液（20%乙二醇 - 丙二醇）可保持细胞和组织的完整性，并提高DNA产量和质量。
