Goto M, Sekine Y, Outa H, Hujikura M, Koichi Suzuki
Department of Bioprocess Engineering, Faculty of Agriculture, Yamagata University, 997-8555, Tsuruoka, Japan
J Insect Physiol. 2001 Feb 1;47(2):157-165. doi: 10.1016/s0022-1910(00)00099-8.
To elucidate the relationship between diapause and cold hardiness in the oriental corn borer, Ostrinia furnacalis, the levels of various substances, cold hardiness and respiration were measured in diapausing and post-diapausing overwintering larvae. Under field conditions, diapause terminated between November and January, although O(2) consumption, measured at 20 degrees C in the laboratory, remained at a high level from October to January. Glycerol content was low during October and November but greatly increased during December and January. Serine was the most abundant of the free amino acids, and its concentrations were especially high during October and November, while the concentration of alanine increased in December and January. Under laboratory conditions, glycerol levels were low in diapausing larvae, and in post-diapausing larvae that were acclimated at either high temperatures or under anaerobic conditions, while they were high in post-diapausing larvae kept under aerobic, low temperature conditions. The survival rate (cold hardiness) was strongly correlated with glycerol content but not with serine or alanine levels. These results suggest that O. furnacalis has a highly developed cold hardiness mechanism in which termination of diapause enables the larvae to increase glycerol levels when the temperature decreases.
为阐明亚洲玉米螟（Ostrinia furnacalis）滞育与耐寒性之间的关系，对滞育和滞育后越冬幼虫体内各种物质的水平、耐寒性及呼吸作用进行了测定。在田间条件下，滞育于11月至1月间结束，尽管在实验室20℃下测定的耗氧量在10月至1月间一直处于较高水平。甘油含量在10月和11月较低，但在12月和1月大幅增加。丝氨酸是含量最丰富的游离氨基酸，其浓度在10月和11月尤其高，而丙氨酸的浓度在12月和1月有所增加。在实验室条件下，滞育幼虫以及在高温或厌氧条件下驯化的滞育后幼虫体内甘油水平较低，而在有氧、低温条件下饲养的滞育后幼虫体内甘油水平较高。存活率（耐寒性）与甘油含量密切相关，而与丝氨酸或丙氨酸水平无关。这些结果表明，亚洲玉米螟具有高度发达的耐寒机制，滞育的结束使幼虫能够在温度下降时提高甘油水平。
