Purać Jelena, Kojić Danijela, Popović Željko D, Vukašinović Elvira, Tiziani Stefano, Günther Ulrich L, Grubor-Lajšić Gordana
Acta Chim Slov. 2015;62(4):761-7.
In this study, an (1)H-NMR -based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to –3 °C and –16 °C, alanine , glycerol and acetate were predominant metabolites. (1)H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.
在本研究中，采用基于氢核磁共振（¹H-NMR）的代谢组学方法，研究欧洲玉米螟滞育幼虫滞育和耐寒性的生化机制。比较了欧洲玉米螟非滞育幼虫和滞育幼虫极性血淋巴提取物中的代谢组学模式。分析确定了13种代谢物：7种氨基酸、甘油、乙酸盐、柠檬酸盐、琥珀酸盐、乳酸盐和腐胺。结果表明，与活跃的非滞育幼虫相比，滞育幼虫呈现出不同的代谢组学模式，主要代谢物为甘油、脯氨酸和丙氨酸。在最初保持在5℃然后逐渐冷却至-3℃和-16℃的特定滞育幼虫中，丙氨酸、甘油和乙酸盐是主要代谢物。¹H-NMR光谱为欧洲玉米螟幼虫抗寒和滞育相关的代谢组学模式提供了新的见解，表明在活跃发育和滞育幼虫中存在不同的代谢组功能。
