Dalbow D G, Young R
Biochem J. 1975 Jul;150(1):13-20. doi: 10.1042/bj1500013.
By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].
通过分析诱导后β-半乳糖苷酶积累的动力学,发现大肠杆菌B/r中β-半乳糖苷酶在快速生长的细胞(每小时1.36和2.1次倍增)中的合成时间为75秒,在缓慢生长的细胞(每小时0.63次倍增)中为90秒。这些值分别对应于每秒16和13个氨基酸的肽链延伸率,与先前的研究结果一致,表明在快速生长的细菌中肽链生长速率是恒定的(可能是最大的),但在缓慢生长的细菌中降低了[福尔哈默和林达尔(1971年)《分子生物学杂志》55卷,563 - 568页]。