大肠杆菌B/r中β-半乳糖苷酶的合成时间与生长速率的关系。
Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate.
作者信息
Dalbow D G, Young R
出版信息
Biochem J. 1975 Jul;150(1):13-20. doi: 10.1042/bj1500013.
Abstract
By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].
摘要
通过分析诱导后β-半乳糖苷酶积累的动力学，发现大肠杆菌B/r中β-半乳糖苷酶在快速生长的细胞（每小时1.36和2.1次倍增）中的合成时间为75秒，在缓慢生长的细胞（每小时0.63次倍增）中为90秒。这些值分别对应于每秒16和13个氨基酸的肽链延伸率，与先前的研究结果一致，表明在快速生长的细菌中肽链生长速率是恒定的（可能是最大的），但在缓慢生长的细菌中降低了[福尔哈默和林达尔（1971年）《分子生物学杂志》55卷，563 - 568页]。
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