大肠杆菌B/r中多肽链延伸速率与生长速率的关系
Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate.
作者信息
Young R, Bremer H
出版信息
Biochem J. 1976 Nov 15;160(2):185-94. doi: 10.1042/bj1600185.
Abstract
By evaluating the kinetics of radioactive labelling of nascent and finished polypeptides, the peptide-chain elongation rate for Escherichia coli B/r at three different growth rates (mu) was determined to be 17 amino acids/s for the fast-growing cells (mu equals 1.3 and 2.0 doublings/h) and 12 amino acids/s for slow-growing cells (mu equals 0.67 doublings/h). The results agree with the growth-rate-dependence of the rate of peptide-chain elongation found for the translation of newly induced beta-galactosidase messenger in this strain and under these conditions of growth [Dalbow & Young (1975) Biochem. J. 150, 13-20]. Together with the previously observed ribosome efficiency at these growth rates [Dennis & Bremer (1974) J. Mol. Biol. 84, 407-422] the results indicate that the fraction of ribosomes engaged in protein synthesis is about 0.8 at all three growth rates.
摘要
通过评估新生多肽和成熟多肽的放射性标记动力学，确定了三种不同生长速率（μ）下大肠杆菌B/r的肽链延伸速率：快速生长的细胞（μ等于1.3和2.0次倍增/小时）为17个氨基酸/秒，缓慢生长的细胞（μ等于0.67次倍增/小时）为12个氨基酸/秒。这些结果与在该菌株中以及在这些生长条件下新诱导的β-半乳糖苷酶信使翻译中发现的肽链延伸速率对生长速率的依赖性一致[Dalbow & Young (1975) Biochem. J. 150, 13 - 20]。结合之前在这些生长速率下观察到的核糖体效率[Dennis & Bremer (1974) J. Mol. Biol. 84, 407 - 422]，结果表明在所有三种生长速率下，参与蛋白质合成的核糖体比例约为0.8。
相似文献
1
Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate.大肠杆菌B/r中多肽链延伸速率与生长速率的关系
Biochem J. 1976 Nov 15;160(2):185-94. doi: 10.1042/bj1600185.
2
Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate.大肠杆菌B/r中β-半乳糖苷酶的合成时间与生长速率的关系。
Biochem J. 1975 Jul;150(1):13-20. doi: 10.1042/bj1500013.
3
Peptide chain elongation rate and ribosomal activity in Saccharomyces cerevisiae as a function of the growth rate.酿酒酵母中肽链延伸速率和核糖体活性与生长速率的关系
Mol Gen Genet. 1979 Feb 26;170(2):225-30. doi: 10.1007/BF00337800.
4
Kinectics of beta-galactosidase synthesis in Escherichia coli at 5 C.大肠杆菌在5摄氏度时β-半乳糖苷酶合成的动力学
J Bacteriol. 1975 Mar;121(3):907-16. doi: 10.1128/jb.121.3.907-916.1975.
5
Role of ribosomal protein S12 in peptide chain elongation: analysis of pleiotropic, streptomycin-resistant mutants of Escherichia coli.核糖体蛋白S12在肽链延伸中的作用：对大肠杆菌多效性、抗链霉素突变体的分析
J Bacteriol. 1977 Mar;129(3):1320-9. doi: 10.1128/jb.129.3.1320-1329.1977.
6
7
Induction kinetics of the L-arabinose operon of Escherichia coli.大肠杆菌L-阿拉伯糖操纵子的诱导动力学
J Bacteriol. 1973 Jul;115(1):9-14. doi: 10.1128/jb.115.1.9-14.1973.
8
Polyamine limitation of growth slows the rate of polypeptide chain elongation in Escherichia coli.多胺对生长的限制会减缓大肠杆菌中多肽链延伸的速率。
J Bacteriol. 1974 Sep;119(3):857-60. doi: 10.1128/jb.119.3.857-860.1974.
9
Control of protein synthesis in Escherichia coli: analysis of an energy source shift-down.大肠杆菌中蛋白质合成的调控：能源转换下调分析
J Bacteriol. 1977 Jul;131(1):18-29. doi: 10.1128/jb.131.1.18-29.1977.
10
Evidence for a high proportion of inactive ribosomes in slow-growing yeast cells.生长缓慢的酵母细胞中存在高比例无活性核糖体的证据。
Biochem J. 1977 Dec 15;168(3):409-15. doi: 10.1042/bj1680409.
引用本文的文献
1
Interpreting ribosome dynamics during mRNA translation.解析mRNA翻译过程中的核糖体动力学
J Biol Chem. 2025 Jul 10;301(8):110469. doi: 10.1016/j.jbc.2025.110469.
2
Ribosomal L12 stalks recruit elongation factors to speed protein synthesis in Escherichia coli.核糖体L12柄招募延伸因子以加速大肠杆菌中的蛋白质合成。
Commun Biol. 2025 Jun 19;8(1):940. doi: 10.1038/s42003-025-08366-4.
3
Single-cell data reveal heterogeneity of investment in ribosomes across a bacterial population.单细胞数据揭示了细菌群体中核糖体投资的异质性。
Nat Commun. 2025 Jan 2;16(1):285. doi: 10.1038/s41467-024-55394-5.
4
The importance of the location of the N-terminus in successful protein folding in vivo and in vitro.N 端位置在体内和体外蛋白质成功折叠中的重要性。
Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2321999121. doi: 10.1073/pnas.2321999121. Epub 2024 Aug 15.
5
How Does Allocate Proteome?蛋白质组如何分配？
ACS Synth Biol. 2024 Sep 20;13(9):2718-2732. doi: 10.1021/acssynbio.3c00537. Epub 2024 Aug 9.
6
Resolving chaperone-assisted protein folding on the ribosome at the peptide level.在肽水平上解析核糖体上伴侣蛋白辅助的蛋白质折叠。
Nat Struct Mol Biol. 2024 Dec;31(12):1888-1897. doi: 10.1038/s41594-024-01355-x. Epub 2024 Jul 10.
7
Understanding and computational design of genetic circuits of metabolic networks.代谢网络遗传电路的理解和计算设计。
Essays Biochem. 2024 Apr 30;68(1):41-51. doi: 10.1042/EBC20230045.
8
Physical modeling of ribosomes along messenger RNA: Estimating kinetic parameters from ribosome profiling experiments using a ballistic model.核糖体沿信使 RNA 的物理建模：使用弹道模型从核糖体图谱实验中估计动力学参数。
PLoS Comput Biol. 2023 Oct 20;19(10):e1011522. doi: 10.1371/journal.pcbi.1011522. eCollection 2023 Oct.
9
Growth-rate dependency of ribosome abundance and translation elongation rate in Corynebacterium glutamicum differs from that in Escherichia coli.在谷氨酸棒杆菌中，核糖体丰度和翻译延伸率的生长速率依赖性与大肠杆菌不同。
Nat Commun. 2023 Sep 12;14(1):5611. doi: 10.1038/s41467-023-41176-y.
10
PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.PKA 调节亚基 Bcy1 在酿酒酵母厌氧木糖生长过程中连接生长、脂质代谢和发酵。
PLoS Genet. 2023 Jul 6;19(7):e1010593. doi: 10.1371/journal.pgen.1010593. eCollection 2023 Jul.
本文引用的文献
1
KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA.诱导酶合成动力学。半乳糖苷酶特异性信使核糖核酸平均寿命的测定。
Biochim Biophys Acta. 1963 Oct 15;76:293-309.
2
Amino acid composition of ribosomes from Escherichia coli.大肠杆菌核糖体的氨基酸组成。
J Mol Biol. 1962 May;4:395-406. doi: 10.1016/s0022-2836(62)80020-5.
3
Peptide chain initiation and growth in the induced synthesis of beta-galactosidase.β-半乳糖苷酶诱导合成中的肽链起始与生长
Biochim Biophys Acta. 1966 Sep;123(3):546-60. doi: 10.1016/0005-2787(66)90222-x.
4
RNA chain growth-rate in Escherichia coli.大肠杆菌中RNA链的生长速率。
J Mol Biol. 1968 Dec 14;38(2):163-80. doi: 10.1016/0022-2836(68)90404-x.
5
Peptide chain growth of -galactosidase in Escherichia coli.大肠杆菌中β-半乳糖苷酶的肽链生长
J Mol Biol. 1968 Jul 14;35(1):165-73. doi: 10.1016/s0022-2836(68)80044-0.
6
Chain elongation rate of messenger and polypeptides in slowly growing Escherichia coli.缓慢生长的大肠杆菌中信使核糖核酸和多肽的链延伸速率
J Mol Biol. 1971 Aug 28;60(1):1-19. doi: 10.1016/0022-2836(71)90442-6.
7
Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15.大肠杆菌15突变体中多肽链生长速率与细胞生长速率的关系
J Mol Biol. 1971 Feb 14;55(3):563-8. doi: 10.1016/0022-2836(71)90337-8.
8
Polypeptide products of nonsense mutations. I. Termination fragments from nonsense mutations in the Z gene of the lac operon of Escherichia coli.无义突变的多肽产物。I. 大肠杆菌乳糖操纵子Z基因无义突变产生的终止片段。
J Mol Biol. 1970 Jun 14;50(2):359-71. doi: 10.1016/0022-2836(70)90198-1.
9
Regulation of ribosomal protein synthesis in Escherichia coli.大肠杆菌中核糖体蛋白合成的调控
J Bacteriol. 1970 Feb;101(2):574-83. doi: 10.1128/jb.101.2.574-583.1970.
10
Differential rate of ribosomal protein synthesis in Escherichia coli B/r.大肠杆菌B/r中核糖体蛋白合成的差异速率。
J Mol Biol. 1974 Apr 15;84(3):407-22. doi: 10.1016/0022-2836(74)90449-5.
Zotero 插件