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细菌中酶诱导作用的分析。

Analysis of enzyme induction in bacteria.

作者信息

Young R, Bremer H

出版信息

Biochem J. 1975 Nov;152(2):243-54. doi: 10.1042/bj1520243.

Abstract

The theoretical relations between the induced initiation and accumulation of lac mRNA and its translation are derived, taking the kinetics of repressor-operator dissociation and enzyme maturation into account. These relations are used to evaluate observed data on lac induction and to estimate a number of parameters that characterize the transcription and translation of the beta-galactosidase gene in the bacterium Escherichia coli B/r growing at three different rates (0.7-2.1 doublings/h).

摘要

考虑到阻遏物 - 操纵基因解离动力学和酶成熟过程,推导了诱导产生的乳糖信使核糖核酸(lac mRNA)的起始与积累及其翻译之间的理论关系。这些关系用于评估乳糖诱导的观测数据,并估计一些表征在三种不同生长速率(0.7 - 2.1次加倍/小时)下生长的大肠杆菌B/r中β - 半乳糖苷酶基因转录和翻译的参数。

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