Sutra L, Risède J M, Gardan L
UMR de Pathologie Végétale INRA-INH-Université d'Angers, Beaucouzé, France.
Lett Appl Microbiol. 2000 Oct;31(4):289-93. doi: 10.1046/j.1472-765x.2000.00816.x.
Total aerobic bacteria and fluorescent pseudomonads were counted in bulk and rhizospheric soils of banana plants of 14 plantations in Martinique (French West Indies). Fluorescent Pseudomonas isolates were then identified and investigated for in vitro antagonism towards Cylindrocladium sp., a fungal pathogen of banana roots. Total aerobic bacteria and fluorescent pseudomonads were significantly more abundant in rhizospheric soils than in bulk soils. Among 58 fluorescent Pseudomonas isolates, 41 were identified as Pseudomonas fluorescens biovar V and 17 as Ps. putida biovar A. Six strains exhibited an antagonism towards Cylindrocladium isolates. Among them, Ps. putida strain 93.1 totally blocked fungal growth. No relationship was established between the antifungal effect and enzyme or hydrogen cyanide production by bacteria, suggesting that siderophores and other compounds were involved in fungal inhibition. Antagonistic fluorescent pseudomonads represent a potential for the biological control of banana root infections by Cylindrocladium sp.
对马提尼克岛（法属西印度群岛）14个种植园香蕉植株的块状土壤和根际土壤中的总需氧细菌和荧光假单胞菌进行了计数。然后对荧光假单胞菌分离株进行鉴定，并研究其对香蕉根真菌病原体柱枝双孢菌的体外拮抗作用。根际土壤中的总需氧细菌和荧光假单胞菌明显比块状土壤中的丰富。在58株荧光假单胞菌分离株中，41株被鉴定为荧光假单胞菌生物变种V，17株为恶臭假单胞菌生物变种A。6株菌株对柱枝双孢菌分离株表现出拮抗作用。其中，恶臭假单胞菌菌株93.1完全抑制了真菌生长。未发现细菌的抗真菌作用与酶或氰化氢产生之间存在关联，这表明铁载体和其他化合物参与了对真菌的抑制。拮抗性荧光假单胞菌具有对柱枝双孢菌引起的香蕉根感染进行生物防治的潜力。
