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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Segal A W, Peters T J

Clin Sci Mol Med. 1975 Dec;49(6):591-6. doi: 10.1042/cs0490591.

A simple quantitative test has been developed to investigate phagocyte function. 2. This test is performed by the addition of Nitroblue Tetrazolium to whole blood, followed by the isolation of leucocytes on a column of nylon wool. Dye reduction by phagocytes is apparent as a blue coloration of the column due to the formation of formazan. The formazan can be extracted from the column and measured spectrophotometrically. 3. The formation of formazan was found to be directly related to the number of phagocytes in blood. 4. Two patients with chronic granulomatous disease gave abnormal results, suggesting that the test procedure may be of value as a screening procedure for this disease.

已开发出一种简单的定量测试来研究吞噬细胞功能。2. 该测试通过向全血中添加硝基蓝四氮唑，然后在尼龙棉柱上分离白细胞来进行。吞噬细胞引起的染料还原表现为由于甲臜的形成，柱呈现蓝色。甲臜可从柱中提取并通过分光光度法进行测量。3. 发现甲臜的形成与血液中吞噬细胞的数量直接相关。4. 两名慢性肉芽肿病患者给出了异常结果，表明该测试程序可能作为这种疾病的筛查程序具有价值。

Segal A W, Peters T J

Clin Sci Mol Med. 1975 Dec;49(6):591-6. doi: 10.1042/cs0490591.

A simple quantitative test has been developed to investigate phagocyte function. 2. This test is performed by the addition of Nitroblue Tetrazolium to whole blood, followed by the isolation of leucocytes on a column of nylon wool. Dye reduction by phagocytes is apparent as a blue coloration of the column due to the formation of formazan. The formazan can be extracted from the column and measured spectrophotometrically. 3. The formation of formazan was found to be directly related to the number of phagocytes in blood. 4. Two patients with chronic granulomatous disease gave abnormal results, suggesting that the test procedure may be of value as a screening procedure for this disease.

已开发出一种简单的定量测试来研究吞噬细胞功能。2. 该测试通过向全血中添加硝基蓝四氮唑，然后在尼龙棉柱上分离白细胞来进行。吞噬细胞引起的染料还原表现为由于甲臜的形成，柱呈现蓝色。甲臜可从柱中提取并通过分光光度法进行测量。3. 发现甲臜的形成与血液中吞噬细胞的数量直接相关。4. 两名慢性肉芽肿病患者给出了异常结果，表明该测试程序可能作为这种疾病的筛查程序具有价值。