Hedley D W, Currie G A
Br J Cancer. 1978 May;37(5):747-52. doi: 10.1038/bjc.1978.113.
Peripheral-blood monocytes from normal individuals and from patients with malignant melanoma reduce nitroblue tetrazolium (NBT). A quantitative assay for dye reduction was applied to 25 healthy donors and 31 patients with malignant melanoma. NBT reduction expressed as dye reduction per monocyte was significantly impaired in patients with disseminated disease, and they responded poorly to a phagocytic stimulus. Monocytes from patients with micrometastatic disease, however, showed normal resting NBT reduction but, following exposure to a suspension of latex-polystyrene, showed significantly greater NBT reduction than those from normal individuals. Since NBT reduction is an indirect measure of intracellular hexose-monophosphate-shunt activity we conclude that the monocytes from patients with minimal disease are in some way activated.
来自正常个体和恶性黑色素瘤患者的外周血单核细胞可使硝基蓝四氮唑（NBT）还原。对25名健康供者和31名恶性黑色素瘤患者进行了染料还原定量检测。以每个单核细胞的染料还原量表示的NBT还原在播散性疾病患者中显著受损，且他们对吞噬刺激反应不佳。然而，微转移疾病患者的单核细胞在静息状态下NBT还原正常，但在接触乳胶聚苯乙烯悬液后，其NBT还原比正常个体的单核细胞显著增强。由于NBT还原是细胞内己糖磷酸旁路活性的间接指标，我们得出结论，疾病处于早期的患者的单核细胞在某种程度上被激活。
