大肠杆菌突变菌株中的脱氧核糖核酸聚合酶II活性
Deoxyribonucleic acid polymerase II activity in an Escherichia coli mutator strain.
作者信息
Smith C L, Shizuya H, Moses R E
出版信息
J Bacteriol. 1976 Jan;125(1):191-6. doi: 10.1128/jb.125.1.191-196.1976.
Abstract
The polB gene encoding deoxyribonucleic acid (DNA) polymerase II has been located close to a mutator gene, mutT1, in Escherichia coli. We find the DNA polymerase II prepared from mutT1, strains to be normal in reaction requirements, heat stability, and ability to remove mismatched bases at termini. Recombinants formed from a mutant defective in DNA polymerase II (polB100) and mutT1 are deficient in polymerase II and have the same mutator phenotype as mutT1. Our linkage analysis indicates that mutT1 and polB100 are not isoallelic.
摘要
编码脱氧核糖核酸（DNA）聚合酶II的polB基因已定位在大肠杆菌中靠近一个诱变基因mutT1的位置。我们发现从mutT1菌株制备的DNA聚合酶II在反应条件、热稳定性以及去除末端错配碱基的能力方面均正常。由DNA聚合酶II缺陷型突变体（polB100）和mutT1形成的重组体缺乏聚合酶II，并且具有与mutT1相同的诱变表型。我们的连锁分析表明mutT1和polB100不是等位基因。
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