大肠杆菌K-12中影响DNA聚合酶II的突变polB100的定位
Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12.
作者信息
Campbell J L, Shizuya H, Richardson C C
出版信息
J Bacteriol. 1974 Aug;119(2):494-9. doi: 10.1128/jb.119.2.494-499.1974.
Abstract
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F(-) crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu(-).
摘要
利用提取物中脱氧核糖核酸聚合酶II的直接检测法，对Hfr×F(-)杂交中的polB等位基因重组体、附加体转移中的F-转导子以及噬菌体P1介导的普遍性转导中的转导子进行筛选。polB基因位于大肠杆菌连锁图谱的2分钟处；它与leu的共转导率为39%至64%。通过P1介导的杂交制备了一种突变体，即大肠杆菌polA1 polB100 polC（ts），该突变体缺乏脱氧核糖核酸聚合酶I和II，并且具有热不稳定的脱氧核糖核酸聚合酶III：P1（HMS85 polB100）×大肠杆菌BT1026 polA1 polC（ts）leu(-)。
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