Theilig C, Tegtmeier Y, Leyhausen G, Geurtsen W
Department of Conservative Dentistry and Periodontology, Medical University, Hannover, Germany.
J Biomed Mater Res. 2000;53(6):632-9. doi: 10.1002/1097-4636(2000)53:6<632::aid-jbm3>3.0.co;2-j.
Previous studies have documented a marked cytotoxic potency of BisGMA and TEGDMA. The purpose of this investigation was to determine if these substances also affect proliferation, migration, and tenascin expression of primary human gingival fibroblasts (HGF) and immortalized human keratinocytes (HaCaT). These parameters play an important role in healing wounds. HGF and HaCaT cultures were incubated with TEGDMA and BisGMA. Cell proliferation (BrdU-assay) and migration (Boyden method) were determined 24 h after incubation. Tenascin expression was investigated four and seven days after treatment. Results were statistically evaluated by ANOVA using the Wilcoxon-Mann-Whitney test (p < 0.05). Proliferation of both cell types was significantly inhibited at concentrations > or = 0.25 mM (TEGDMA) or > or = 0.01 mM (BisGMA). Migration of HaCaT was significantly increased after incubation with BisGMA for 24 h. TEGDMA did not alter migration of HGF and HaCaT. In addition, TEGDMA had no effect on tenascin expression of both cell cultures. After 4 days of incubation, BisGMA (at a concentration of 0.01 mM) significantly reduced tenascin production of HaCaT cultures related to cell number. However, 7 days after treatment, BisGMA significantly increased tenascin expression of HGF and HaCaT cultures. Altogether, our results indicate that BisGMA can affect migration of keratinocytes and alters the expression of the extracellular matrix component tenascin. Thus, BisGMA may significantly influence the healing of injured oral tissues.
先前的研究已证明BisGMA和TEGDMA具有显著的细胞毒性。本研究的目的是确定这些物质是否也会影响原代人牙龈成纤维细胞（HGF）和永生化人角质形成细胞（HaCaT）的增殖、迁移和腱生蛋白表达。这些参数在伤口愈合中起着重要作用。将HGF和HaCaT培养物与TEGDMA和BisGMA一起孵育。孵育24小时后测定细胞增殖（BrdU检测）和迁移（Boyden法）。在处理后第4天和第7天研究腱生蛋白表达。结果采用方差分析和Wilcoxon-Mann-Whitney检验进行统计学评估（p<0.05）。当浓度≥0.25 mM（TEGDMA）或≥0.01 mM（BisGMA）时，两种细胞类型的增殖均受到显著抑制。与BisGMA孵育24小时后，HaCaT的迁移显著增加。TEGDMA未改变HGF和HaCaT的迁移。此外，TEGDMA对两种细胞培养物的腱生蛋白表达均无影响。孵育4天后，BisGMA（浓度为0.01 mM）与细胞数量相关地显著降低了HaCaT培养物中腱生蛋白的产生。然而，处理7天后，BisGMA显著增加了HGF和HaCaT培养物中腱生蛋白的表达。总之，我们的结果表明BisGMA可影响角质形成细胞的迁移并改变细胞外基质成分腱生蛋白的表达。因此，BisGMA可能会显著影响受损口腔组织的愈合。
