约旦北部因呼吸道疾病住院儿童中通过空斑试验瓶增强呼吸道合胞病毒检测

Enhanced detection of respiratory syncytial virus by shell vial in children hospitalised with respiratory illnesses in northern Jordan.

作者信息

Meqdam M M, Nasrallah G K

机构信息

Departments of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan.

出版信息

J Med Virol. 2000 Dec;62(4):518-23. doi: 10.1002/1096-9071(200012)62:4<518::aid-jmv18>3.0.co;2-p.

DOI:10.1002/1096-9071(200012)62:4<518::aid-jmv18>3.0.co;2-p

PMID:11074482

Abstract

During the period between November 1997 and May 1998, a total of 350 nasopharyngeal aspirates were obtained from children admitted to the Respiratory Disease Unit at Princess Rahma Hospital, northern Jordan, and diagnosed clinically as suffering from respiratory tract infections. Nasopharyngeal aspirates were investigated for the presence of respiratory syncycial virus (RSV) by using shell vial (SV) culture assay, conventional culture assay, and direct immunofluorescence assay. Out of 350 nasopharyngeal aspirates, 101(28.9%) were found positive by any of SV, conventional culture, and immunofluorescence; 91 (90.1%) by SV, 87(86.1%) by culture, and 83(82.2%) by immunofluorescence. The maximum number of virus isolations was noted in children below the age of 1 year and was associated with bronchiolitis. SV assay showed the highest sensitivity (94.3%) and specificity (96.9%) for detecting RSV from nasopharyngeal aspirates. These results emphasise the importance of SV culture assay for diagnosis of RSV, although immunofluorescence is a valuable, rapid diagnostic assay.

摘要

在1997年11月至1998年5月期间，从约旦北部拉赫玛公主医院呼吸疾病科收治的临床诊断为患有呼吸道感染的儿童中，共采集了350份鼻咽抽吸物。采用空斑小室（SV）培养试验、传统培养试验和直接免疫荧光试验对鼻咽抽吸物进行呼吸道合胞病毒（RSV）检测。在350份鼻咽抽吸物中，通过SV、传统培养和免疫荧光中的任何一种方法检测发现101份（28.9%）呈阳性；通过SV检测呈阳性的有91份（90.1%），通过培养检测呈阳性的有87份（86.1%），通过免疫荧光检测呈阳性的有83份（82.2%）。1岁以下儿童的病毒分离数量最多，且与细支气管炎有关。SV试验在从鼻咽抽吸物中检测RSV时显示出最高的敏感性（94.3%）和特异性（96.9%）。这些结果强调了SV培养试验对RSV诊断的重要性，尽管免疫荧光是一种有价值的快速诊断试验。