利用改进型蛋白质测序仪和质谱法对大肠杆菌核糖体大亚基中14种蛋白质的一级结构进行的研究。

Studies on the primary structure of 14 proteins from the large subunit of Escherichia coli ribosomes with an improved protein sequenator and with mass spectrometry.

作者信息

Wittmann-Liebold B, Geissler A W, Marzinzig E

出版信息

J Supramol Struct. 1975;3(5-6):426-47. doi: 10.1002/jss.400030505.

Abstract

Fourteen proteins from the large subunit of Escherichia coli ribosomes were analyzed in an improved sequenator. In addition to our previously described modifications of a Beckman sequenator, new valves which work free of a dead volume were constructed. By this and the previous improvements (e.g., a new vacuum system with a recorder, cool traps, automatic conversion) much better results were obtained than before. It was even possible to use (in addition to the standard methods, e.g., thin-layer chromatography and amino acid analysis) mass spectrometry without preceding gas chromatography for identification of the released PTH amino acids. Our experience with the various methods, especially mass spectrometry, is described and the techniques are compared. The results obtained by the described methods on the amino acid sequences of the 14 ribosomal proteins are summarized.

摘要

在一台改进的测序仪中对大肠杆菌核糖体大亚基的14种蛋白质进行了分析。除了我们之前描述的对贝克曼测序仪的改进外，还构建了无死体积的新型阀门。通过这一改进以及之前的改进措施（例如，带有记录仪、冷阱、自动转换功能的新型真空系统），获得了比以前更好的结果。甚至可以（除了标准方法，如薄层色谱法和氨基酸分析外）在不进行气相色谱的情况下使用质谱法来鉴定释放的PTH氨基酸。描述了我们在各种方法，尤其是质谱法方面的经验，并对这些技术进行了比较。总结了通过所述方法获得的14种核糖体蛋白质氨基酸序列的结果。