Yusof N L, Lim L Y, Khor E
Department of Chemistry, National University of Singapore, Kent Ridge, Singapore 119260.
J Biomed Mater Res. 2001 Jan;54(1):59-68. doi: 10.1002/1097-4636(200101)54:1<59::aid-jbm7>3.0.co;2-u.
Chitin was dissolved in N, N-dimethylacetamide/5% lithium chloride (DMAc/5%LiCl) to form a 0.5% chitin solution. Chitin beads were formed by dropping the 0.5% chitin solution into a nonsolvent coagulant, ethanol. The beads were left in ethanol for 24 h to permit hardening, consolidation, and removal of residual DMAc/5%LiCl solvent in order to give spherical chitin beads uniform size distribution. The ethanol-gelled chitin beads had an average diameter of 535 microm. The chitin beads were subsequently activated in 50% (w/v) NaOH solution and reacted with 1.9 M monochloroacetic acid/2-propanol solution to introduce a carboxymethylated surface layer to the chitin beads. The bilayer character of the surface-carboxymethylated chitin (SCM-chitin) beads was verified by Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and confocal microscopy. The bilayered SCM-chitin beads were found to absorb up to 95 times their dry weight of water. These SCM-chitin beads have potential as a component of wound dressings.
将几丁质溶解于N，N - 二甲基乙酰胺/5%氯化锂（DMAc/5%LiCl）中，形成0.5%的几丁质溶液。通过将0.5%的几丁质溶液滴入非溶剂凝固剂乙醇中来形成几丁质珠粒。将珠粒留在乙醇中24小时，以使其硬化、固结并去除残留的DMAc/5%LiCl溶剂，从而得到尺寸分布均匀的球形几丁质珠粒。乙醇凝胶化的几丁质珠粒的平均直径为535微米。随后将几丁质珠粒在50%（w/v）的氢氧化钠溶液中活化，并与1.9 M的一氯乙酸/2 - 丙醇溶液反应，在几丁质珠粒表面引入羧甲基化表层。通过傅里叶变换红外（FTIR）光谱、热重分析（TGA）和共聚焦显微镜对表面羧甲基化几丁质（SCM - 几丁质）珠粒的双层特性进行了验证。发现双层SCM - 几丁质珠粒能够吸收高达其干重95倍的水。这些SCM - 几丁质珠粒有潜力作为伤口敷料的一个成分。
