Muzzarelli Riccardo A A, Guerrieri Mario, Goteri Gaia, Muzzarelli Corrado, Armeni Tatiana, Ghiselli Roberto, Cornelissen Maria
Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Ancona, Italy.
Biomaterials. 2005 Oct;26(29):5844-54. doi: 10.1016/j.biomaterials.2005.03.006. Epub 2005 Apr 26.
Dibutyryl chitin (DBC) is a modified chitin carrying butyryl groups at 3 and 6 positions; its peculiarity is that it dissolves promptly in common solvents, while being insoluble in aqueous systems. The high biocompatibility of dibutyryl chitin in the form of films and non-wovens has been demonstrated for human, chick and mouse fibroblasts by the Viability/Cytotoxicity assay, In situ Cell Proliferation assay, Neutral Red Retention assay, Lactate Dehydrogenase Release assay, MTS cytotoxicity assay, and scanning electron microscopy. DBC was hardly degradable by lysozyme, amylase, collagenase, pectinase and cellulase over the observation period of 48 days at room temperature, during which no more than 1.33% by weight of the DBC filaments (0.3 mm diameter) was released to the aqueous medium. DBC non-wovens were incorporated into 5-methylpyrrolidinone chitosan solution and submitted to freeze-drying to produce a reinforced wound dressing material. The latter was tested in vivo in full thickness wounds in rats. The insertion of 4x4 mm pieces did not promote any adverse effect on the healing process, as shown histologically. DBC is therefore suitable for contacting intact and wounded human tissues.
二丁酰壳聚糖（DBC）是一种在3位和6位带有丁酰基的改性壳聚糖；其独特之处在于它能迅速溶解于常见溶剂中，而不溶于水体系。通过活力/细胞毒性测定、原位细胞增殖测定、中性红保留测定、乳酸脱氢酶释放测定、MTS细胞毒性测定以及扫描电子显微镜，已证明二丁酰壳聚糖以薄膜和无纺布形式对人、鸡和小鼠成纤维细胞具有高度生物相容性。在室温下48天的观察期内，DBC几乎不能被溶菌酶、淀粉酶、胶原酶、果胶酶和纤维素酶降解，在此期间，直径0.3毫米的DBC细丝释放到水介质中的重量不超过1.33%。将DBC无纺布加入5-甲基吡咯烷酮壳聚糖溶液中，然后进行冷冻干燥，以生产一种增强型伤口敷料材料。后者在大鼠的全层伤口中进行了体内测试。组织学结果表明，插入4×4毫米的小块对愈合过程没有任何不良影响。因此，DBC适合与完整的和受伤的人体组织接触。
