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在铜绿假单胞菌中导入含有噬菌体D3粘性末端位点的质粒。

Transduction of a plasmid containing the bacteriophage D3 cos site in Pseudomonas aeruginosa.

作者信息

Sharp R, Gertman E, Farinha M A, Kropinski A M

机构信息

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

出版信息

J Bacteriol. 1990 Jun;172(6):3509-11. doi: 10.1128/jb.172.6.3509-3511.1990.

DOI:10.1128/jb.172.6.3509-3511.1990
PMID:2111812
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209169/
Abstract

Plasmids harboring the cos sequences of bacteriophage D3 can be transferred, by bacteriophage D3, into Pseudomonas aeruginosa by a mechanism which is insensitive to DNase. Transducing activity was separated from the plaque-forming particles by CsCl equilibrium gradient centrifugation. Restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers.

摘要

携带噬菌体D3 cos序列的质粒可通过噬菌体D3，以一种对DNA酶不敏感的机制转移到铜绿假单胞菌中。通过氯化铯平衡梯度离心将转导活性与噬菌斑形成颗粒分离。限制性内切酶消化模式表明转导颗粒含有质粒串联体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e905/209169/9c6c39bb7bb4/jbacter00160-0704-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e905/209169/9c6c39bb7bb4/jbacter00160-0704-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e905/209169/9c6c39bb7bb4/jbacter00160-0704-a.jpg

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Exonuclease activity from Pseudomonas aeruginosa which is missing in phenotypically restrictionless mutants.铜绿假单胞菌的核酸外切酶活性在表型无限制突变体中缺失。
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