Jung E, Heller M, Sanchez J C, Hochstrasser D F
Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland.
Electrophoresis. 2000 Oct;21(16):3369-77. doi: 10.1002/1522-2683(20001001)21:16<3369::AID-ELPS3369>3.0.CO;2-7.
Proteome research aims to unravel the biological complexity encoded by the genome. Due to the complexity of higher eukaryotic cells, single-step characterization of a proteome is likely to be difficult to achieve. However, advantage can be taken of the macromolecular architecture of a cell, e.g., subcellular compartments, organelles, macromolecular structures and multiprotein complexes, to establish subcellular proteomes. This review highlights recent developments in this area of proteomics, namely the establishment of two-dimensional electrophoresis (2-DE) reference maps of subcellular compartments and organelles as well as the characterization of macromolecular structures and multiprotein complexes using a proteomics approach.
蛋白质组研究旨在揭示基因组所编码的生物复杂性。由于高等真核细胞的复杂性，对蛋白质组进行单步表征可能难以实现。然而，可以利用细胞的大分子结构，如亚细胞区室、细胞器、大分子结构和多蛋白复合物，来建立亚细胞蛋白质组。本综述重点介绍了蛋白质组学这一领域的最新进展，即建立亚细胞区室和细胞器的二维电泳（2-DE）参考图谱，以及使用蛋白质组学方法对大分子结构和多蛋白复合物进行表征。
