Linde B, Chisolm G
Acta Physiol Scand. 1975 Dec;95(4):383-90. doi: 10.1111/j.1748-1716.1975.tb10065.x.
The interstitial 14C-sucrose space was determined in the subsutaneous adipose tissue of the dog using both a single injection-indicator dilution technique and an equilibration-tissue sampling method. 131I-albumin and 51Cr-labelled erythrocytes served as intravascular indicators. The conventional extrapolation method for single injection curves yielded space values several times lower than those measured by the equilibration technique, although sampling was continued until the venous outflow samples contained less than 1% of the peak 14C-sucrose activity. This discrepancy may be due to long transit times, for which the conventional extrapolation procedure does not account. An extrapolation procedure is proposed based on the assumption that the area under the normalized extra- and intravascular curves should be equal. By this procedure as well as by the equilibration technique the interstitial 14C-sucrose space in adipose tissue measures approximately 10 mlX100 g-1. The single injection technique does not appear applicable for space determinations in adipose tissue during sympathetic nerve stimulation (3-7 Hz), possibly due to "trapping" of the tracers within the tissue.
采用单次注射-指示剂稀释技术和平衡-组织采样法测定了犬皮下脂肪组织中的间质14C-蔗糖空间。131I-白蛋白和51Cr标记的红细胞用作血管内指示剂。尽管采样持续到静脉流出样本中14C-蔗糖活性低于峰值的1%，但单次注射曲线的传统外推法得出的空间值比平衡技术测得的值低几倍。这种差异可能是由于转运时间长，而传统外推程序未考虑这一点。基于血管外和血管内标准化曲线下面积应相等的假设，提出了一种外推程序。通过该程序以及平衡技术，脂肪组织中的间质14C-蔗糖空间约为10 ml×100 g-1。单次注射技术似乎不适用于交感神经刺激（3-7 Hz）期间脂肪组织中的空间测定，这可能是由于示踪剂在组织内“滞留”所致。
