van Keulen L J, Langeveld J P, Garssen G J, Jacobs J G, Schreuder B E, Smits M A
Institute for Animal Science and Health, ID-Lelystad, The Netherlands.
Vet Q. 2000 Oct;22(4):197-200. doi: 10.1080/01652176.2000.9695057.
Cows affected with bovine spongiform encephalopathy (BSE) display chronic neurological signs consisting of behavioural changes, abnormalities of posture and movement, and/or hyperaesthesia. At present, there are no laboratory test available to diagnose BSE in the live animal. In this article, we describe the post-mortem diagnostic examination of brains from BSE-suspected cattle as currently performed at ID-Lelystad. The routine laboratory diagnosis of BSE consists of histopathological examination of the brain and detection of the modified prion protein, PrP(BSE), in brain tissue. These tests, however, have the disadvantage of being laborious and time consuming, so that results are available only after several days. Recently, at ID-Lelystad a new post-mortem test has been developed that enables screening of larger volumes of brain samples for PrP(BSE) within 1 day. This BSE test is especially suited for slaughterline monitoring. A preliminary validation study has shown that both sensitivity and specificity are 100% compared to the gold diagnostic standard of histopathology.
感染牛海绵状脑病（BSE）的奶牛表现出慢性神经症状，包括行为改变、姿势和运动异常，以及/或者感觉过敏。目前，尚无用于在活体动物中诊断BSE的实验室检测方法。在本文中，我们描述了目前在莱利斯塔德传染病研究所对疑似患有BSE的牛进行的脑死后诊断检查。BSE的常规实验室诊断包括对脑进行组织病理学检查以及检测脑组织中的变异朊病毒蛋白PrP（BSE）。然而，这些检测方法存在费力且耗时的缺点，以至于结果要在数天后才能获得。最近，莱利斯塔德传染病研究所开发了一种新的死后检测方法，能够在1天内对大量脑样本进行PrP（BSE）筛查。这种BSE检测方法特别适用于屠宰线监测。一项初步验证研究表明，与组织病理学的金标准诊断相比，其灵敏度和特异性均为1
