Mathioudakis G, Storb R, McSweeney P A, Torok-Storb B, Lansdorp P M, Brümmendorf T H, Gass M J, Bryant E M, Storek J, Flowers M E, Gooley T, Nash R A
Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
Blood. 2000 Dec 1;96(12):3991-4.
Donor-derived hematopoiesis was assessed in 17 patients who received allogeneic marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood counts were normal or near normal in all patients except one. Chimerism analyses, using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) or analysis of variable number tandem repeat loci, indicated that 15 out of 16 patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had indeterminate chimerism. All 12 recipients of grafts from female donors exhibited polyclonal hematopoiesis by X-linked clonal analysis with the use of molecular probes. Of the 17 recipients, 9 exhibited a less than 1.0-kilobase shortening of granulocyte telomere length compared with their respective donors, according to terminal restriction fragment analysis or flow-FISH with a fluorescein-labeled peptide nucleic acid probe. These data suggest that under standard transplantation conditions, the stem cell proliferative potential is not compromised during hematopoietic reconstitution. (Blood. 2000;96:3991-3994)
对1971年至1980年间接受来自HLA匹配同胞的异基因骨髓移植的17例患者进行了供体来源造血的评估。除1例患者外，所有患者的全血细胞计数均正常或接近正常。使用双色XY染色体荧光原位杂交（FISH）或可变数目串联重复序列位点分析进行的嵌合分析表明，16例患者中有15例供体来源造血大于97%，而1例患者的嵌合情况不确定。通过使用分子探针的X连锁克隆分析，所有12例接受女性供体移植的受者均表现出多克隆造血。根据末端限制片段分析或使用荧光素标记肽核酸探针的流式FISH，17例受者中有9例与各自供体相比，粒细胞端粒长度缩短小于1.0千碱基。这些数据表明，在标准移植条件下，造血重建过程中干细胞增殖潜能未受损害。（《血液》。2000年；96:3991 - 3994）
