Hasegawa T, Noda K, Kumamoto S, Ando Y, Yamada A, Yoshikai Y
Research Laboratories, Chlorella Industry Co. Ltd, 1343 Hisatomi, Chikugo City, 833-0056, Fukuoka, Japan.
Int J Immunopharmacol. 2000 Nov;22(11):877-85. doi: 10.1016/s0192-0561(00)00049-7.
A glycoprotein prepared from Chlorella vulgaris culture supernatant (CVS) is a biological response modifier (BRM) which exhibits protective activities against tumor metastasis and 5-fluorouracil-induced immunosuppression. We here show that oral administration of CVS prevented significantly the apoptosis of thymocytes in mice undergoing psychological stress in a communication box. Mice were exposed to the emotional stress for 14 days by witnessing other mice being exposed to foot-shock. The numbers in thymocytes, especially CD4(+)CD8(+) population, were decreased significantly and apoptotic cells, as assessed by Annexin V expression, were reciprocally increased after the exposure to the psychological stress. C. vulgaris culture supernatant (CVS) administration significantly suppressed the increase in serum corticosterone level in the psychologically stressed mice. These results suggest that CVS prevents psychological stress and maintain homeostasis in the face of external environmental changes.
从普通小球藻培养上清液（CVS）中制备的一种糖蛋白是一种生物反应调节剂（BRM），它具有抗肿瘤转移和5-氟尿嘧啶诱导的免疫抑制的保护活性。我们在此表明，口服CVS可显著预防在交流箱中经历心理应激的小鼠胸腺细胞的凋亡。通过目睹其他小鼠遭受足部电击，使小鼠暴露于情绪应激14天。胸腺细胞数量，尤其是CD4(+)CD8(+)群体，显著减少，并且通过膜联蛋白V表达评估，暴露于心理应激后凋亡细胞相应增加。给予普通小球藻培养上清液（CVS）可显著抑制心理应激小鼠血清皮质酮水平的升高。这些结果表明，CVS可预防心理应激并在面对外部环境变化时维持体内稳态。
