Tanaka K, Yamada A, Noda K, Hasegawa T, Okuda M, Shoyama Y, Nomoto K
Research Laboratories, Chlorella Industries Co. Ltd., Fukuoka, Japan.
Cancer Immunol Immunother. 1998 Feb;45(6):313-20. doi: 10.1007/s002620050448.
A glycoprotein extract (CVS), derived from the unicellular green alga Chlorella vulgaris, strain CK22, exhibited a pronounced antitumor effect against both spontaneous and experimentally induced metastasis in mice. Inhibition of tumor metastasis was enhanced when intratumor administration of CVS was followed by s.c. injection of CVS. Anti-metastatic immunopotentiation was observed in euthymic mice, but not in athymic nude mice. The antitumor activity of CVS was reflected in antigen-specific, T-cell-mediated immunity. Both CD4 and CD8 T cells contributed to the antimetastatic effects, as shown by in vivo depletion experiments with anti-T-cell subset antibodies. Furthermore, CVS caused the recruitment of T cells to the regional lymph nodes and their proliferation in these organs. The CD4-positive population, following CVS injection at the time of tumor rechallenge, displayed a pronounced increase in the proportion of T cells that were CD18 bright, CD44 bright, CD25+, CD54+, CD69+ or CD71+ in the lymph nodes. Thus, CVS induces T cell activation in peripheral lymph nodes in tumor-bearing mice. We conclude that CVS augments antimetastatic immunity through T cell activation in lymphoid organs and enhances recruitment of these cells to the tumor sites. Presurgical treatment with CVS might prevent metastasis or tumor progression.
从普通小球藻（绿藻门单细胞藻类）CK22株提取的糖蛋白提取物（CVS），对小鼠自发性转移和实验诱导转移均显示出显著的抗肿瘤作用。当瘤内注射CVS后再进行皮下注射CVS时，肿瘤转移的抑制作用增强。在正常胸腺小鼠中观察到抗转移免疫增强作用，但在无胸腺裸鼠中未观察到。CVS的抗肿瘤活性体现在抗原特异性的T细胞介导的免疫反应中。体内用抗T细胞亚群抗体进行耗竭实验表明，CD4和CD8 T细胞均有助于抗转移作用。此外，CVS可使T细胞募集到局部淋巴结并在这些器官中增殖。在肿瘤再次攻击时注射CVS后，CD4阳性群体在淋巴结中显示出CD18高表达、CD44高表达、CD25 +、CD54 +、CD69 +或CD71 +的T细胞比例显著增加。因此，CVS可诱导荷瘤小鼠外周淋巴结中的T细胞活化。我们得出结论，CVS通过激活淋巴器官中的T细胞增强抗转移免疫力，并增强这些细胞向肿瘤部位的募集。术前用CVS治疗可能预防转移或肿瘤进展。
