胞饮作用的定量研究。I. 体外培养的大鼠卵黄囊对（125I）聚乙烯吡咯烷酮的摄取动力学
Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.
作者信息
Williams K E, Kidston E M, Beck F, Lloyd J B
出版信息
J Cell Biol. 1975 Jan;64(1):113-22. doi: 10.1083/jcb.64.1.113.
Abstract
A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.
摘要
本文描述了一种17.5天龄大鼠内脏卵黄囊的体外培养方法。通过光学显微镜和电子显微镜判断，组织存活良好。该组织对125I标记的聚乙烯吡咯烷酮的胞饮摄取率在实验内和实验间均保持恒定。在0.15 - 24微克/毫升的浓度范围内，125I标记的聚乙烯吡咯烷酮既不刺激也不抑制胞饮作用。该系统在胞饮作用物理基础的定量研究中具有许多优势。
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