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胞饮作用的定量研究。I. 体外培养的大鼠卵黄囊对（125I）聚乙烯吡咯烷酮的摄取动力学

Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.

作者信息

Williams K E, Kidston E M, Beck F, Lloyd J B

出版信息

J Cell Biol. 1975 Jan;64(1):113-22. doi: 10.1083/jcb.64.1.113.

DOI:10.1083/jcb.64.1.113
PMID:1109230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109486/
Abstract

A method is described for the in vitro culture of 17.5-day rat visceral yolk sac. Tissue survival was good as judged by light and electron microscopy. The rate of pinocytic uptake of 125I-labeled polyvinylpyrrolidone by the tissue was constant both within and between experiments. Within the concentration range 0.15-24 mug/ml, the 125I-labeled polyvinylpyrrolidone neither stimulated nor inhibited pinocytosis. The system offers many advantages in the quantitative study of the physical basis of pinocytosis.

摘要

本文描述了一种17.5天龄大鼠内脏卵黄囊的体外培养方法。通过光学显微镜和电子显微镜判断，组织存活良好。该组织对125I标记的聚乙烯吡咯烷酮的胞饮摄取率在实验内和实验间均保持恒定。在0.15 - 24微克/毫升的浓度范围内，125I标记的聚乙烯吡咯烷酮既不刺激也不抑制胞饮作用。该系统在胞饮作用物理基础的定量研究中具有许多优势。

相似文献

1
Quantitative studies of pinocytosis. I. Kinetics of uptake of (125I)polyvinylpyrrolidone by rat yolk sac cultured in vitro.胞饮作用的定量研究。I. 体外培养的大鼠卵黄囊对（125I）聚乙烯吡咯烷酮的摄取动力学
J Cell Biol. 1975 Jan;64(1):113-22. doi: 10.1083/jcb.64.1.113.
2
Quantitative studies of pinocytosis. II. Kinetics of protein uptake and digestion by rat yolk sac cultured in vitro.胞饮作用的定量研究。II. 体外培养的大鼠卵黄囊摄取和消化蛋白质的动力学
J Cell Biol. 1975 Jan;64(1):123-34. doi: 10.1083/jcb.64.1.123.
3
Inhibition of pinocytosis in rat yolk sac by trypan blue.
Teratology. 1976 Dec;14(3):343-54. doi: 10.1002/tera.1420140310.
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A quantitative study of pinocytosis and lysosome function in experimentally induced lysosomal storage.实验性诱导溶酶体贮积中胞饮作用和溶酶体功能的定量研究
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Adsorptive pinocytosis of polycationic copolymers of vinylpyrrolidone with vinylamine by rat yolk sac and rat peritoneal macrophage.大鼠卵黄囊和大鼠腹腔巨噬细胞对乙烯基吡咯烷酮与乙烯胺的聚阳离子共聚物的吸附性胞饮作用。
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Adsorptive pinocytosis of 125I-labelled lactate dehydrogenase isoenzymes H4 and M4 by rat yolk sacs incubated in vitro.体外培养的大鼠卵黄囊对125I标记的乳酸脱氢酶同工酶H4和M4的吸附性胞饮作用。
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Quantitative studies of pinocytic uptake of 125I-labelled polyvinylpyrrolidone by pig aortic smooth-muscle cells in culture.对培养的猪主动脉平滑肌细胞摄取¹²⁵I标记的聚乙烯吡咯烷酮的胞饮作用的定量研究。
Biochem Soc Trans. 1977;5(1):130-3. doi: 10.1042/bst0050130.
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Pinocytosis in the rat visceral yolk sac. Effects of temperature, metabolic inhibitors and some other modifiers.大鼠内脏卵黄囊中的胞饮作用。温度、代谢抑制剂及其他一些调节剂的影响。
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Inhibition of rat yolk sac pinocytosis by cadmium and its reversal by zinc.镉对大鼠卵黄囊胞饮作用的抑制及其锌逆转作用
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The effect of chemical treatments of albumin and orosomucoid on rate of clearance from the rat bloodstream and rate of pinocytic capture of rat yolk sac cultured in vitro.白蛋白和类粘蛋白的化学处理对其从大鼠血液循环中清除速率以及体外培养的大鼠卵黄囊胞饮摄取速率的影响。
Biochem J. 1977 Jun 15;164(3):607-16. doi: 10.1042/bj1640607.

引用本文的文献

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An in vitro study of teratogenicity in the rat due to antibody-induced yolk sac dysfunction : Identification of the yolk sac antigen involved.抗体诱导的卵黄囊功能障碍导致大鼠致畸性的体外研究：相关卵黄囊抗原的鉴定。
Rouxs Arch Dev Biol. 1986 May;195(4):236-242. doi: 10.1007/BF02438956.
2
Comparative immunochemistry and ontogeny of two closely related coated pit proteins. The 280-kd target of teratogenic antibodies and the 330-kd target of nephritogenic antibodies.两种密切相关的被膜小窝蛋白的比较免疫化学与个体发生。致畸抗体的280-kd靶标和致肾炎抗体的330-kd靶标。
Am J Pathol. 1993 May;142(5):1654-67.
3
Antibodies to the 280-kd coated pit protein, target of teratogenic antibodies, produce alterations in the traffic of internalized proteins.针对280kd包被小窝蛋白（致畸抗体的靶标）的抗体，会使内化蛋白的运输发生改变。
Am J Pathol. 1994 Dec;145(6):1526-36.
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Effects of weak bases on the degradation of endogenous and exogenous proteins by rat yolk sacs.弱碱对大鼠卵黄囊降解内源性和外源性蛋白质的影响。
Biochem J. 1980 Jun 15;188(3):895-903. doi: 10.1042/bj1880895.
5
The effect of sodium salicylate on the rat embryo in culture: an in vitro model for the morphological assessment of teratogenicity.水杨酸钠对培养的大鼠胚胎的影响：一种用于致畸性形态学评估的体外模型。
J Anat. 1981 Sep;133(Pt 2):257-69.
6
Exocytosis of pinocytosed fluid in cultured cells: kinetic evidence for rapid turnover and compartmentation.培养细胞中胞饮液的胞吐作用：快速周转和区室化的动力学证据。
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7
Adsorptive pinocytosis of 125I-labelled lactate dehydrogenase isoenzymes H4 and M4 by rat yolk sacs incubated in vitro.体外培养的大鼠卵黄囊对125I标记的乳酸脱氢酶同工酶H4和M4的吸附性胞饮作用。
Biochem J. 1981 Sep 15;198(3):587-93. doi: 10.1042/bj1980587.
8
Effect of molecular size of 125I-labelled poly(vinylpyrrolidone) on its pinocytosis by rat visceral yolk sacs and rat peritoneal macrophages.¹²⁵I标记的聚乙烯吡咯烷酮分子大小对大鼠内脏卵黄囊和大鼠腹腔巨噬细胞胞饮作用的影响
Biochem J. 1981 Apr 15;196(1):49-55. doi: 10.1042/bj1960049.
9
Fluid endocytosis by rat liver and spleen. Experiments with 125I-labelled poly(vinylpyrrolidone) in vivo.大鼠肝脏和脾脏的液体胞吞作用。体内用125I标记的聚（乙烯基吡咯烷酮）进行的实验。
Biochem J. 1980 Nov 15;192(2):613-21. doi: 10.1042/bj1920613.
10
The renal excretion and retention of macromolecules: The chemical structure effect.大分子的肾脏排泄与潴留：化学结构效应
Pflugers Arch. 1982 Jan;392(3):211-7. doi: 10.1007/BF00584298.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
DIFFERENTIAL UPTAKE OF PROTEIN AND GLUCOSE BY PINOCYTOSIS IN AMOEBA PROTEUS.变形虫通过胞饮作用对蛋白质和葡萄糖的差异摄取
C R Trav Lab Carlsberg. 1964;34:211-26.
3
A histochemical and biochemical study of some aspects of placental function in the rat using maternal injection of horseradish peroxidase.通过给母鼠注射辣根过氧化物酶对大鼠胎盘功能某些方面进行的组织化学和生物化学研究。
J Anat. 1967 Jun;101(Pt 3):461-78.
4
Lysosomal enzyme inhibition by trypan blue: a theory of teratogenesis.锥虫蓝对溶酶体酶的抑制作用：一种致畸理论。
Science. 1967 Sep 8;157(3793):1180-2. doi: 10.1126/science.157.3793.1180.
5
Kinetics of pinocytosis.胞饮作用的动力学
Fed Proc. 1967 Jul-Aug;26(4):987-93.
6
The regulation of pinocytosis in mouse macrophages. 3. The induction of vesicle formation by nucleosides and nucleotides.小鼠巨噬细胞中胞饮作用的调节。3. 核苷和核苷酸对囊泡形成的诱导作用。
J Exp Med. 1967 Mar 1;125(3):457-66. doi: 10.1084/jem.125.3.457.
7
Effects of triton WR-1339 on the rat yolk sac placenta.曲拉通WR-1339对大鼠卵黄囊胎盘的影响。
Am J Anat. 1966 Sep;119(2):199-233. doi: 10.1002/aja.1001190203.
8
Development of structure and function in the mammalian yolk sac. I. Developmental morphology and vitamin B12 uptake of the rat yolk sac.哺乳动物卵黄囊的结构与功能发育。I. 大鼠卵黄囊的发育形态学及维生素B12摄取
Dev Biol. 1966 Jun;13(3):311-48. doi: 10.1016/0012-1606(66)90053-4.
9
An electron microscopic visualization of transport across rat visceral yolk sac.大鼠内脏卵黄囊转运的电子显微镜观察
Am J Anat. 1966 Jan;118(1):21-52. doi: 10.1002/aja.1001180104.
10
Pinocytic uptake of ferritin by the amoeba Chaos chaos measured by atomic absorption of iron.通过铁的原子吸收法测定变形虫大变形虫对铁蛋白的胞饮摄取。
C R Trav Lab Carlsberg. 1970;38(2):19-57.