Baysan A, Whiley R A, Lynch E
Department of Conservative Dentistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK.
Caries Res. 2000 Nov-Dec;34(6):498-501. doi: 10.1159/000016630.
The aims of this present study were (1) to assess the antimicrobial effect of ozone from a novel ozone-generating device (Heolozone, USA) [0.052% (v/v) in air delivered at a rate of 13.33 ml.s(-1)] on primary root carious lesions (PRCLs) and (2) to evaluate the efficacy of ozone specifically on Streptococcus mutans and Streptococcus sobrinus. In study 1, 40 soft PRCLs from freshly extracted teeth were randomly divided into two groups to test the antimicrobial effect on PRCLs from exposure to ozonated water for either 10 or 20 s. Half of a lesion was removed using a sterile excavator. Subsequently, the remaining lesion was exposed to the ozonised water for a period of either 10 or 20 s (corresponding to 0. 069 or 0.138 ml of ozone, respectively). Using paired Student t tests, a significant (p<0.001) reduction (mean +/- SE) was observed in the ozone-treated groups with either a 10-second (log(10) 3.57+/-0.37) or 20-second (log(10) 3.77+/-0.42) ozone application compared with the control groups (log(10) 5.91+/-0.15 and log(10) 6.18+/-0.21, respectively). In study 2, 40 sterile saliva-coated glass beads were randomly divided into two groups for each micro-organism. One glass bead was put into each bijou bottle with 3 ml of Todd-Hewitt broth. S. mutans and S. sobrinus were inoculated anaerobically overnight. Each glass bead was then washed with 2 ml of phosphate-buffered saline. Immediately, 10 s of ozone gas was applied to each glass bead in the test groups. There was a significant (p<0.0001) reduction (mean +/- SE) in ozone-treated samples for S. mutans (log(10) 1.01+/-0.27) and S. sobrinus (log(10) 1.09+/-0.36) compared with the control samples (log(10) 3.93+/-0.07 and log(10) 4.61+/-0.13, respectively). This treatment regime is an effective, quick, conservative and simple method to kill micro-organisms in PRCLs. Ozone gas application for a period of 10 s was also capable of reducing the numbers of S. mutans and S. sobrinus on saliva-coated glass beads in vitro.
（1）评估一种新型臭氧发生装置（美国Heolozone）产生的臭氧[空气中含量为0.052%（v/v），输送速率为13.33 ml·s⁻¹]对原发性根龋损害（PRCLs）的抗菌效果；（2）具体评估臭氧对变形链球菌和远缘链球菌的疗效。在研究1中，将40个从新鲜拔除牙齿上获取的软质PRCLs随机分为两组，以测试暴露于臭氧水10秒或20秒对PRCLs的抗菌效果。用无菌挖匙去除一半的损害组织。随后，将剩余的损害组织暴露于臭氧水10秒或20秒（分别对应0.069或0.138 ml的臭氧）。使用配对学生t检验，与对照组相比（对照组分别为log₁₀5.91±0.15和log₁₀6.18±0.21），臭氧处理组在应用10秒（log₁₀3.57±0.37）或20秒（log₁₀3.77±0.42）臭氧后观察到显著（p<0.001）减少（平均值±标准误）。在研究2中，针对每种微生物将40个无菌唾液包被的玻璃珠随机分为两组。将一个玻璃珠放入每个装有3 ml Todd-Hewitt肉汤的比色瓶中。变形链球菌和远缘链球菌厌氧接种过夜。然后每个玻璃珠用2 ml磷酸盐缓冲盐水洗涤。立即对测试组中的每个玻璃珠施加10秒的臭氧气体。与对照样品相比（对照样品分别为log₁₀3.93±0.07和log₁₀4.61±0.13），臭氧处理的变形链球菌样品（log₁₀1.01±0.27）和远缘链球菌样品（log₁₀1.09±0.36）有显著（p<0.0001）减少（平均值±标准误）。这种治疗方案是一种有效、快速、保守且简单的杀死PRCLs中微生物的方法。应用10秒的臭氧气体也能够在体外减少唾液包被玻璃珠上变形链球菌和远缘链球菌的数量。
