粟酒裂殖酵母tRNA：假尿苷合酶Pus1p的克隆与特性分析
Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p.
作者信息
Hellmuth K, Grosjean H, Motorin Y, Deinert K, Hurt E, Simos G
机构信息
BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
出版信息
Nucleic Acids Res. 2000 Dec 1;28(23):4604-10. doi: 10.1093/nar/28.23.4604.
Abstract
Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A Schizosaccharomyces pombe gene, named spPUS1, was cloned from a cDNA library by complementation of this conditional lethal phenotype. The corresponding protein, spPus1p, shows sequence similarity to S. cerevisiae and murine Pus1p as well as other known members of the pseudouridine synthase family. Accordingly, recombinant spPus1p can catalyze in vitro the formation of pseudouridines at positions 27, 28, 34, 35 and 36 of yeast tRNA transcripts. The sequence and functional conservation of the Pus1p proteins in fungi and mammalian species and their notable absence from prokaryotes suggest that this family of pseudouridine synthases is required for a eukaryote-specific step of tRNA biogenesis, such as nuclear export.
摘要
在LOS1（一种tRNA输出受体）和PUS1（一种tRNA：假尿苷合酶）基因中均带有缺失的酿酒酵母细胞表现出温度敏感型生长缺陷。通过对这种条件致死表型的互补作用，从一个cDNA文库中克隆出了粟酒裂殖酵母的一个名为spPUS1的基因。相应的蛋白质spPus1p与酿酒酵母和小鼠的Pus1p以及假尿苷合酶家族的其他已知成员具有序列相似性。因此，重组的spPus1p能够在体外催化酵母tRNA转录本27、28、34、35和36位假尿苷的形成。真菌和哺乳动物物种中Pus1p蛋白的序列和功能保守性以及它们在原核生物中明显不存在，这表明该假尿苷合酶家族是tRNA生物合成中真核生物特有的步骤（如核输出）所必需的。
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本文引用的文献
1
An aminoacylation-dependent nuclear tRNA export pathway in yeast.酵母中一种依赖氨酰化的细胞核tRNA输出途径。
Genes Dev. 2000 Apr 1;14(7):830-40.
2
Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of psi(2819) in 21S mitochondrial ribosomal RNA.酿酒酵母中负责在21S线粒体核糖体RNA中形成假尿苷（ψ2819）的RNA：假尿苷合酶的鉴定。
Nucleic Acids Res. 2000 May 1;28(9):1941-6. doi: 10.1093/nar/28.9.1941.
3
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I.假尿苷合酶I识别tRNA并形成假尿苷的结构基础。
Nat Struct Biol. 2000 Jan;7(1):23-7. doi: 10.1038/71219.
4
The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA.从假尿苷合酶I与5-氟尿嘧啶-tRNA的相互作用推导其作用机制。
Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14270-5. doi: 10.1073/pnas.96.25.14270.
5
Point mutations in yeast CBF5 can abolish in vivo pseudouridylation of rRNA.酵母CBF5中的点突变可消除rRNA在体内的假尿苷化修饰。
Mol Cell Biol. 1999 Nov;19(11):7461-72. doi: 10.1128/MCB.19.11.7461.
6
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J Mol Biol. 1999 Jun 11;289(3):491-502. doi: 10.1006/jmbi.1999.2789.
7
Coordination of tRNA nuclear export with processing of tRNA.tRNA核输出与tRNA加工的协调
RNA. 1999 Apr;5(4):539-49. doi: 10.1017/s1355838299982134.
8
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9
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Mol Cell Biol. 1999 Mar;19(3):2142-54. doi: 10.1128/MCB.19.3.2142.
10
The role of exportin-t in selective nuclear export of mature tRNAs.输出蛋白t在成熟tRNA选择性核输出中的作用。
EMBO J. 1998 Dec 15;17(24):7430-41. doi: 10.1093/emboj/17.24.7430.
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