Matos H R, Di Mascio P, Medeiros M H
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil.
Arch Biochem Biophys. 2000 Nov 1;383(1):56-9. doi: 10.1006/abbi.2000.2035.
A high incidence of cancer has been correlated with chronic iron overload, and carotenoids are of interest as possible anticarcinogens. We have investigated the effect of lycopene on lipid peroxidation and on the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in CV1-P monkey cells exposed to ferric nitrilotriacetate (Fe-NTA) plus ascorbate. Cells supplemented with lycopene (20 pmol/10(6) cells) showed a reduction of 86% in Fe-NTA/ascorbate-induced lipid peroxidation (TBARS). Levels of 8-oxodGuo rose from 1.59+/-0.09 residues/10(6) dGuo in the control cells to 14.02+/-0.41 residues/10(6) dGuo after incubation with (1:4 mM) Fe-NTA/ascorbate (40 microM). Lycopene supplementation decreased in 77% the 8-oxodGuo levels in Fe-NTA/ascorbate-treated cells. These results indicate that lycopene can protect mammalian cells against membrane and DNA damage and possibly play a protective role against tumor promotion associated with oxidative damage.
癌症的高发病率与慢性铁过载相关，而类胡萝卜素作为可能的抗癌物质受到关注。我们研究了番茄红素对暴露于次氮基三乙酸铁（Fe-NTA）加抗坏血酸的CV1-P猴细胞中脂质过氧化和8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代dGuo）形成的影响。补充番茄红素（20 pmol/10⁶个细胞）的细胞在Fe-NTA/抗坏血酸诱导的脂质过氧化（硫代巴比妥酸反应物）方面降低了86%。在与（1:4 mM）Fe-NTA/抗坏血酸（40 μM）孵育后，对照细胞中8-氧代dGuo的水平从1.59±0.09个残基/10⁶个脱氧鸟苷增加到14.02±0.41个残基/10⁶个脱氧鸟苷。补充番茄红素使Fe-NTA/抗坏血酸处理细胞中的8-氧代dGuo水平降低了77%。这些结果表明，番茄红素可以保护哺乳动物细胞免受膜和DNA损伤，并可能在预防与氧化损伤相关的肿瘤促进方面发挥保护作用。
