使用基于小亚基核糖体RNA的诊断和基因分型工具鉴定暴雨水中隐孢子虫卵囊的种类和来源。
Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool.
作者信息
Xiao L, Alderisio K, Limor J, Royer M, Lal A A
机构信息
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Chamblee, Georgia 30341, USA.
出版信息
Appl Environ Microbiol. 2000 Dec;66(12):5492-8. doi: 10.1128/AEM.66.12.5492-5498.2000.
Abstract
The identification of Cryptosporidium oocysts in environmental samples is largely made by the use of an immunofluorescent assay. In this study, we have used a small-subunit rRNA-based PCR-restriction fragment length polymorphism technique to identify species and sources of Cryptosporidium oocysts present in 29 storm water samples collected from a stream in New York. A total of 12 genotypes were found in 27 positive samples; for 4 the species and probable origins were identified by sequence analysis, whereas the rest represent new genotypes from wildlife. Thus, this technique provides an alternative method for the detection and differentiation of Cryptosporidium parasites in environmental samples.
摘要
环境样本中隐孢子虫卵囊的鉴定主要通过免疫荧光检测法进行。在本研究中，我们使用了基于小亚基核糖体RNA的聚合酶链反应-限制性片段长度多态性技术，来鉴定从纽约一条溪流采集的29份雨水样本中存在的隐孢子虫卵囊的种类和来源。在27份阳性样本中总共发现了12种基因型；通过序列分析鉴定出其中4种的种类和可能来源，其余则代表来自野生动物的新基因型。因此，该技术为环境样本中隐孢子虫寄生虫的检测和鉴别提供了一种替代方法。
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