Wang J, Li G, Lu H, Zheng Z, Huang Y, Su W
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell, Engineering and School of Life Sciences, Xiamen University, 361005, Xiamen, PR China.
FEMS Microbiol Lett. 2000 Dec 15;193(2):249-53. doi: 10.1111/j.1574-6968.2000.tb09432.x.
The diterpenoid taxol is an important anticancer agent used widely in the clinic. The purpose of this work was to identify a taxol-producing endophytic fungus (strain TF5) isolated from Taxus mairei and study its anticancer activities. Strain TF5 was identified as a Tubercularia sp. according to the morphology of the fungal culture, the mechanism of spore production and the characteristics of the spores. Strain TF5 produced taxol, when grown in potato dextrose liquid medium and analyzed by thin layer chromatography, high performance liquid chromatography, ultraviolet and mass spectrometry. The fungal taxol, which was isolated from the organic extract of the TF5 culture, had strong cytotoxic activity towards KB and P388 cancer cells in vitro, tested by the MTT assay. Observed with immunofluorescence and electron microscopy, the fungal taxol enhanced microtubule stability and bundling in culture cells and induced tubulin polymerization in vitro similar to the authentic taxol.
二萜类化合物紫杉醇是一种重要的抗癌药物，在临床上广泛应用。本研究的目的是鉴定从南方红豆杉中分离出的一株产紫杉醇的内生真菌（菌株TF5），并研究其抗癌活性。根据真菌培养物的形态、孢子产生机制和孢子特征，菌株TF5被鉴定为瘤座孢属（Tubercularia sp.）。当菌株TF5在马铃薯葡萄糖液体培养基中生长，并通过薄层色谱、高效液相色谱、紫外光谱和质谱分析时，可产生紫杉醇。通过MTT法检测，从TF5培养物的有机提取物中分离得到的真菌紫杉醇在体外对KB和P388癌细胞具有很强的细胞毒性活性。通过免疫荧光和电子显微镜观察，真菌紫杉醇增强了培养细胞中微管的稳定性和成束，并在体外诱导微管蛋白聚合，类似于天然紫杉醇。
