Bentsink L, Alonso-Blanco C, Vreugdenhil D, Tesnier K, Groot S P, Koornneef M
Laboratory of Genetics, Graduate School Experimental Plant Science, Wageningen University, 2 Dreijenlaan 6703 HA Wageningen, The Netherlands.
Plant Physiol. 2000 Dec;124(4):1595-604. doi: 10.1104/pp.124.4.1595.
Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance and consequently in seed storability. In the present work we analyzed the seed-soluble OS (sucrose, raffinose, and stachyose) content of several Arabidopsis accessions and thus identified the genotype Cape Verde Islands having a very low RSO content. By performing quantitative trait loci (QTL) mapping in a recombinant inbred line population, we found one major QTL responsible for the practically monogenic segregation of seed stachyose content. This locus also affected the content of the two other OSs, sucrose, and raffinose. Two candidate genes encoding respectively for galactinol synthase and raffinose synthase were located within the genomic region around this major QTL. In addition, three smaller-effect QTL were identified, each one specifically affecting the content of an individual OS. Seed storability was analyzed in the same recombinant inbred line population by measuring viability (germination) under two different seed aging assays: after natural aging during 4 years of dry storage at room temperature and after artificial aging induced by a controlled deterioration test. Thus, four QTL responsible for the variation of this trait were mapped. Comparison of the QTL genetic positions showed that the genomic region containing the major OS locus did not significantly affect the seed storability. We concluded that in the studied material neither RSOs nor sucrose content had a specific effect on seed storability.
种子寡糖（OSs），尤其是棉子糖系列寡糖（RSOs），被认为在获得脱水耐受性从而在种子耐贮性方面发挥重要作用。在本研究中，我们分析了几种拟南芥生态型种子中的可溶性OS（蔗糖、棉子糖和水苏糖）含量，从而鉴定出一种RSO含量极低的基因型——佛得角群岛。通过在重组自交系群体中进行数量性状基因座（QTL）定位，我们发现了一个主要的QTL，它导致种子水苏糖含量几乎呈单基因分离。该基因座还影响了另外两种OS（蔗糖和棉子糖）的含量。在这个主要QTL周围的基因组区域内定位了两个分别编码半乳糖醇合酶和棉子糖合酶的候选基因。此外，还鉴定出了三个效应较小的QTL，每个QTL特异性地影响一种单独OS的含量。通过在两种不同的种子老化试验（在室温下干燥储存4年的自然老化和通过控制劣变试验诱导的人工老化）下测量活力（发芽率），对同一重组自交系群体的种子耐贮性进行了分析。因此，定位了四个负责该性状变异的QTL。QTL遗传位置的比较表明，包含主要OS基因座的基因组区域对种子耐贮性没有显著影响。我们得出结论，在所研究的材料中，RSOs和蔗糖含量对种子耐贮性均没有特定影响。
