拟南芥中25个干旱诱导基因（RD和ERD）的定位
Mapping of 25 drought-inducible genes, RD and ERD, in Arabidopsis thaliana.
作者信息
Taji T, Seki M, Yamaguchi-Shinozaki K, Kamada H, Giraudat J, Shinozaki K
机构信息
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibaraki, Japan.
出版信息
Plant Cell Physiol. 1999 Jan;40(1):119-23. doi: 10.1093/oxfordjournals.pcp.a029469.
We mapped 25 Arabidopsis thaliana drought-inducible genes. Responsive to Dehydration (RD) and Early Responsive to Dehydration (ERD), to the Arabidopsis genome and compared map positions with those of mutants that show environmental stress response. We hybridized CIC yeast artificial chromosome (YAC) library filters with the cDNAs and determined the map positions of 18 corresponding genes. We screened the P1 library with 7 other clones and analyzed segregation of their restriction fragment length polymorphisms (RFLP) in recombinant inbred (RI) lines. One cDNA could be mapped because it had been sequenced by the Arabidopsis genome sequencing program.
我们将25个拟南芥干旱诱导基因，即脱水响应基因（RD）和早期脱水响应基因（ERD），定位到拟南芥基因组上，并将图谱位置与那些表现出环境胁迫响应的突变体的图谱位置进行比较。我们用这些互补脱氧核糖核酸（cDNA）与CIC酵母人工染色体（YAC）文库滤膜杂交，并确定了18个相应基因的图谱位置。我们用另外7个克隆筛选P1文库，并分析了它们的限制性片段长度多态性（RFLP）在重组自交（RI）系中的分离情况。由于一个cDNA已由拟南芥基因组测序计划测序，所以它能够被定位。
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