Fraser N W, Sehgal P B, Darnell J E
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2571-5. doi: 10.1073/pnas.76.6.2571.
Discrete-sized short RNA chains that contain the distinctive oligonucleotides, including the 5'-capped oligonucleotide, characteristic of the first 600 nucleotides of the adenovirus type 2 (Ad-2) large, late, rightward-reading transcriptional unit (16.4-99) accumulate in Ad-2-infected HeLa cells. In the presence of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole the accumulation of these chains is enhanced, as is the accumulation of short chains from a neighbouring rightward-reading transcriptional unit (3.0-10.7). These short chains appear to represent prematurely terminated transcripts. Late in infection there is a marked increase in RNA synthesis, including that of prematurely terminated short chains, from the large late transcriptional unit. This suggests that the increase in transcription and mRNA production from this region late in infection is not due to reduced "attenuation" of RNA synthesis.
包含独特寡核苷酸的离散大小的短RNA链，包括5'-帽化寡核苷酸，其具有腺病毒2型（Ad-2）大的、晚期、向右阅读转录单位（16.4 - 99）前600个核苷酸的特征，在Ad-2感染的HeLa细胞中积累。在5,6-二氯-1-β-D-呋喃核糖基苯并咪唑存在的情况下，这些链的积累以及来自相邻向右阅读转录单位（3.0 - 10.7）的短链的积累都会增强。这些短链似乎代表过早终止的转录本。在感染后期，来自大的晚期转录单位的RNA合成显著增加，包括过早终止的短链的合成。这表明感染后期该区域转录和mRNA产生的增加并非由于RNA合成的“衰减”减少所致。
