Kim S S, Gallaher D D, Csallany A S
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.
Lipids. 2000 Nov;35(11):1225-37. doi: 10.1007/s11745-000-0639-2.
Diabetes mellitus is characterized by complications affecting several organs, including the kidney. Lipid peroxidation increases in diabetes and has been implicated in the pathogenesis of diabetic complications. In this study, we examined the ability of two antioxidants, vitamin E and probucol, to reduce lipid peroxidation in vivo and renal hypertrophy, an early stage of diabetic nephropathy, in rats. Animals were divided into four groups: non-diabetic, diabetic, diabetic treated with vitamin E, and diabetic treated with probucol. Animals were given antioxidants by intraperitoneal injection after induction of diabetes by streptozotocin injection. After 7 wk, lipid peroxidation in vivo was measured by analyzing urinary excretion of lipophilic aldehydes and related carbonyl compounds (LACC) as 2,4-dinitrophenylhydrazones by high-performance liquid chromatography. A number of urinary lipophilic nonpolar and polar aldehydes and related carbonyl compounds were identified, almost all of which increased in diabetes. Antioxidant treatment resulted in significantly decreased excretion of urinary LACC excretion. Antioxidant treatment of diabetic rats reduced renal hypertrophy. There was a high correlation between kidney weight and urinary LACC. Since LACC are accepted markers of lipid peroxidation, these results indicate that antioxidants can reduce the elevated lipid peroxidation of diabetes and may slow the onset of diabetic nephropathy.
糖尿病的特征是会引发影响多个器官（包括肾脏）的并发症。糖尿病患者体内脂质过氧化增加，这与糖尿病并发症的发病机制有关。在本研究中，我们检测了两种抗氧化剂——维生素E和普罗布考——在体内降低脂质过氧化以及减轻大鼠肾肥大（糖尿病肾病的早期阶段）的能力。将动物分为四组：非糖尿病组、糖尿病组、维生素E治疗糖尿病组和普罗布考治疗糖尿病组。通过注射链脲佐菌素诱导糖尿病后，经腹腔注射给予动物抗氧化剂。7周后，通过高效液相色谱法分析亲脂性醛类及相关羰基化合物（LACC）作为2,4-二硝基苯腙的尿排泄量，来测定体内脂质过氧化情况。鉴定出了多种尿亲脂性非极性和极性醛类及相关羰基化合物，几乎所有这些物质在糖尿病患者体内都有所增加。抗氧化剂治疗导致尿LACC排泄量显著降低。对糖尿病大鼠进行抗氧化剂治疗可减轻肾肥大。肾脏重量与尿LACC之间存在高度相关性。由于LACC是脂质过氧化公认的标志物，这些结果表明抗氧化剂可以降低糖尿病患者升高的脂质过氧化水平，并可能延缓糖尿病肾病的发病。
