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快速检测幽门螺杆菌23S rRNA基因中的突变，这些突变使该细菌对克拉霉素治疗产生耐药性。

Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium.

作者信息

Matsumura M, Hikiba Y, Ogura K, Togo G, Tsukuda I, Ushikawa K, Shiratori Y, Omata M

机构信息

The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.

出版信息

J Clin Microbiol. 2001 Feb;39(2):691-5. doi: 10.1128/JCM.39.2.691-695.2001.

DOI:10.1128/JCM.39.2.691-695.2001
PMID:11158129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87798/
Abstract

We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.

摘要

我们开发了一种新方法，能够使用LightCycler检测幽门螺杆菌23S rRNA基因中的点突变。我们的方法可以在不到1小时内检测出该基因中的突变，并且可以一次性处理多个样本，从而有助于选择适合基于克拉霉素治疗的患者。

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Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium.快速检测幽门螺杆菌23S rRNA基因中的突变，这些突变使该细菌对克拉霉素治疗产生耐药性。
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本文引用的文献

1
Detection of clarithromycin-resistant helicobacter pylori strains by a preferential homoduplex formation assay.通过优先同源双链形成试验检测耐克拉霉素幽门螺杆菌菌株
J Clin Microbiol. 2000 Jan;38(1):210-4. doi: 10.1128/JCM.38.1.210-214.2000.
2
Simultaneous colonisation of Helicobacter pylori with and without mutations in the 23S rRNA gene in patients with no history of clarithromycin exposure.在无克拉霉素暴露史的患者中，同时存在23S rRNA基因有突变和无突变的幽门螺杆菌定植。
Gut. 1999 Oct;45(4):503-7. doi: 10.1136/gut.45.4.503.
3
High-speed prothrombin G-->A 20210 and methylenetetrahydrofolate reductase C-->T 677 mutation detection using real-time fluorescence PCR and melting curves.运用实时荧光定量聚合酶链反应和熔解曲线进行凝血酶原G20210A和亚甲基四氢叶酸还原酶C677T突变的快速检测。
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Occurence of resistance mutation and clonal expansion in Helicobacter pylori multiple-strain infection: a potential risk in clarithromycin-based therapy.
Clin Infect Dis. 1999 Jun;28(6):1305-7. doi: 10.1086/514796.
5
Multiplex sequence analysis demonstrates the competitive growth advantage of the A-to-G mutants of clarithromycin-resistant Helicobacter pylori.多重序列分析证明了耐克拉霉素幽门螺杆菌A到G突变体的竞争生长优势。
Antimicrob Agents Chemother. 1999 Mar;43(3):683-5. doi: 10.1128/AAC.43.3.683.
6
Helicobacter pylori specific nested PCR assay for the detection of 23S rRNA mutation associated with clarithromycin resistance.用于检测与克拉霉素耐药相关的23S rRNA突变的幽门螺杆菌特异性巢式PCR检测法。
Gut. 1998 Sep;43(3):317-21. doi: 10.1136/gut.43.3.317.
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Clarithromycin-resistant Helicobacter pylori in patients with duodenal ulcer in the United States.美国十二指肠溃疡患者中对克拉霉素耐药的幽门螺杆菌
Am J Gastroenterol. 1998 Sep;93(9):1432-5. doi: 10.1111/j.1572-0241.1998.455_t.x.
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Genotypic characterization of clarithromycin-resistant and -susceptible Helicobacter pylori strains from the same patient demonstrates existence of two unrelated isolates.对来自同一患者的克拉霉素耐药和敏感幽门螺杆菌菌株进行基因分型，结果表明存在两种不相关的分离株。
J Clin Microbiol. 1998 Sep;36(9):2730-1. doi: 10.1128/JCM.36.9.2730-2731.1998.
9
Identification of a 23S rRNA gene mutation in clarithromycin-resistant Helicobacter pylori.耐克拉霉素幽门螺杆菌中23S rRNA基因突变的鉴定
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