Hiscott P, Magee R M, Colthurst M, Lois N, Wong D
Unit of Ophthalmology, Department of Medicine, University of Liverpool, Liverpool, UK.
Br J Ophthalmol. 2001 Feb;85(2):179-83. doi: 10.1136/bjo.85.2.179.
BACKGROUND/AIMS: Epiretinal and retrolental proliferation may occur during prolonged use of the novel tamponade agent perfluorohexyloctane (F(6)H(8)). This study aims to determine whether there is any histological evidence that F(6)H(8) has a role in the formation of these membranes.
Eight epiretinal membranes and three opaque posterior lens capsules were excised from patients in whom F(6)H(8) had been used as a long term retinal tamponade agent. The membranes and capsules were examined employing light microscopic methods, including immunohistochemistry.
The epiretinal membranes showed histological features typical of proliferative vitreoretinopathy (PVR) epiretinal membranes, but they also exhibited a dense macrophagic infiltration. In addition, three of the membranes contained multinucleated cells. Macrophages represented up to 30% of the cells present and appeared to contain large intracytoplasmic vacuoles. Similar cells were seen on the back of the posterior lens capsule in one specimen and all three capsules had posterior migration of lens epithelium.
The pathological findings are not simply those of PVR. The macrophage infiltration suggests that there may be a biological reaction to F(6)H(8) which could reflect its surmised propensity to emulsify. Further investigations concerning the cellular response to this promising tamponade agent are warranted.
背景/目的：在长期使用新型填塞剂全氟己基辛烷（F(6)H(8)）期间，可能会发生视网膜前和晶状体后增殖。本研究旨在确定是否有组织学证据表明F(6)H(8)在这些膜的形成中起作用。
从长期使用F(6)H(8)作为视网膜填塞剂的患者中切除8个视网膜前膜和3个不透明的晶状体后囊。采用包括免疫组织化学在内的光学显微镜方法对这些膜和囊进行检查。
视网膜前膜表现出增殖性玻璃体视网膜病变（PVR）视网膜前膜的典型组织学特征，但也表现出密集的巨噬细胞浸润。此外，其中3个膜含有多核细胞。巨噬细胞占存在细胞的30%，似乎含有大的胞质内空泡。在一个标本的晶状体后囊背面可见类似细胞，所有3个囊均有晶状体上皮细胞向后迁移。
病理结果并非单纯的PVR表现。巨噬细胞浸润表明可能对F(6)H(8)有生物学反应，这可能反映了其推测的乳化倾向。有必要对这种有前景的填塞剂的细胞反应进行进一步研究。
