Umlas J
Transfusion. 1975 Mar-Apr;15(2):111-5. doi: 10.1046/j.1537-2995.1975.15275122803.x.
In order to determine the maximum degree to which blood units could be packed and still be effective, shelf stored blood and previously frozen red blood cells were washed and hyperpacked to hematocrits of 90 to 98 per cent. These products had average volumes of 180 and 162 ml, respectively. When transfused into a group of patients with stable nonhemolytic anemias or with slow or intermittent blood loss, the hyperpacked shelf stored blood resulted in average hematocrit increments of 4.4 per cent; the hyperpacked frozen red blood cells resulted in average hematocrit increments of 3.4 per cent. Conventionally packed unwashed red blood cells had approximately 10 per cent more hemoglobin and volumes of 270 to 330 ml, but resulted in average hematocrit elevations of only 2.8 per cent. Unwashed blood hyperpacked to hematocrits of 90 per cent with removal of the visible buffy coat took much longer to administer. Thus, by washing and hyperpacking shelf stored blood or previously forzen red blood cells, transfusions with the minimal amount of extraneous material can be given.
为了确定血液单位能够被浓缩到何种最大程度且仍保持有效性，对库存血液和先前冷冻的红细胞进行洗涤并超浓缩至血细胞比容为90%至98%。这些产品的平均体积分别为180毫升和162毫升。当将这些超浓缩的库存血液输注给一组患有稳定的非溶血性贫血或有缓慢或间歇性失血的患者时，血细胞比容平均升高4.4%；超浓缩的冷冻红细胞使血细胞比容平均升高3.4%。传统包装的未洗涤红细胞的血红蛋白含量大约多10%，体积为270至330毫升，但血细胞比容平均仅升高2.8%。去除可见的白膜层并超浓缩至血细胞比容为90%的未洗涤血液输注耗时长得多。因此，通过洗涤和超浓缩库存血液或先前冷冻的红细胞，可以用最少的外来物质进行输血。
