Carini M, Aldini G, Furlanetto S, Stefani R, Facino R M
Istituto Chimico Farmaceutico Tossicologico, Milano, Italy.
J Pharm Biomed Anal. 2001 Jan;24(3):517-26. doi: 10.1016/s0731-7085(00)00431-3.
Liquid Chromatography-Ion Trap Mass Spectrometry with an atmospheric pressure chemical ionization (APCI) interface in the negative and positive-ion modes in parallel to UV-diode-array detection (DAD), was applied for the rapid detection/characterization in crude extracts of the water-soluble antioxidant phenolics from Helichrysum stoechas. APCI-MS provides unequivocal molecular weight data of these compounds and useful information about their structures (diagnostic fragments ions), which were confirmed by the UV-DAD fingerprints. This combined approach allows the identification of ten constituents, including the three naturally occurring isomers of caffeoylquinic acid (CGAs), namely neo-chlorogenic acid, chlorogenic acid and crypto-chlorogenic acid, 2 isomeric dicaffeoyl quinic acids, 2 isomeric naringenin glucosides, quercetin, kaempferol and apigenin glucosides and a tetrahydroxychalcone-glucoside. The water-soluble extract from H. stoechas, standardized in both total polyphenol and kaempferol-3-glucoside content, exhibits strong antioxidant activity in vitro when tested in both artificial membrane systems (phosphatidylcholine liposomes) and in a cell model (rat erythrocytes).
采用液相色谱-离子阱质谱联用技术，配备大气压化学电离（APCI）接口，可在负离子和正离子模式下并行操作，并结合紫外二极管阵列检测（DAD），用于快速检测/表征蜡菊粗提物中的水溶性抗氧化酚类物质。APCI-MS可提供这些化合物明确的分子量数据及其结构的有用信息（诊断性碎片离子），这些信息通过UV-DAD指纹图谱得到证实。这种联合方法可鉴定出十种成分，包括咖啡酰奎宁酸（CGAs）的三种天然异构体，即新绿原酸、绿原酸和隐绿原酸，两种异构的二咖啡酰奎宁酸，两种异构的柚皮苷葡萄糖苷，槲皮素、山奈酚和芹菜素葡萄糖苷以及一种四羟基查耳酮葡萄糖苷。蜡菊的水溶性提取物在总多酚和山奈酚-3-葡萄糖苷含量方面均已标准化，在人工膜系统（磷脂酰胆碱脂质体）和细胞模型（大鼠红细胞）中进行测试时，均表现出很强的体外抗氧化活性。
