Stroka J, Van Otterdijk R, Anklam E
Joint Research Centre of the European Commission, Institute for Health and Consumer Protection, Ispra, Italy
J Chromatogr A. 2000 Dec 29;904(2):251-6. doi: 10.1016/s0021-9673(00)00930-4.
A one-dimensional TLC method to determine aflatoxins (B1, B2, G1, G2) in various food matrices was elaborated which abstains fully on the use of chlorinated solvents. It implements an immunoaffinity clean-up step after extraction with methanol. The aflatoxins were quantified by densitometry. The method has shown to be rapid and efficient. In-house performance characteristics were established. The limit of quantification was found to be significantly lower than current regulatory limits for aflatoxin control outside and within the European Community. The obtained recovery and precision data gave a strong indication, that the method is likely to give satisfactory performance if tested in a future collaborative trial.
阐述了一种一维薄层色谱法，用于测定各种食品基质中的黄曲霉毒素（B1、B2、G1、G2），该方法完全避免使用氯化溶剂。在用甲醇萃取后，它采用免疫亲和净化步骤。黄曲霉毒素通过光密度法进行定量。该方法已证明快速有效。建立了内部性能特征。发现定量限显著低于欧盟内外黄曲霉毒素控制的现行监管限值。所获得的回收率和精密度数据有力地表明，如果在未来的协同试验中进行测试，该方法可能会有令人满意的表现。
