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人博卡病毒1对Caco-2细胞系培养物的感染。

Human bocavirus 1 infection of CACO-2 cell line cultures.

作者信息

Ghietto Lucía María, Toigo D'Angelo Ana Paola, Viale Franco Agustin, Adamo María Pilar

机构信息

Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

Instituto de Virología "Dr. J. M. Vanella", Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

出版信息

Virology. 2017 Oct;510:273-280. doi: 10.1016/j.virol.2017.07.034. Epub 2017 Aug 1.

DOI:10.1016/j.virol.2017.07.034
PMID:28777951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7172243/
Abstract

Human bocavirus 1 (HBoV1) is a parvovirus associated with pneumonia in infants. It has been detected in different tissues, including colorectal tumors. In this study, we investigated whether Caco-2 cell line, derived from human colon cancer, can be utilized as a model for HBoV1 replication. We demonstrate HBoV1 replication in Caco-2 cultures supplemented with DEAE-dextran after inoculation with respiratory material from infected patients presenting with acute respiratory infection. A viral cycle of rapid development is displayed. However, in spite of HBoV1 DNA 4-fold increment in the supernatants and monolayers by day 1, evidencing that the system allows the virus genome replication after the entry occurred, infectious progeny particles were not produced. These results are consistent with an infection that is limited to a single growth cycle, which can be associated to mutations in the NS1 and VP1/VP2 regions of HBoV1 genome. Further research will contribute to fully elucidate these observations.

摘要

人博卡病毒1型（HBoV1）是一种与婴儿肺炎相关的细小病毒。它已在包括结肠直肠肿瘤在内的不同组织中被检测到。在本研究中，我们调查了源自人结肠癌的Caco-2细胞系是否可作为HBoV1复制的模型。我们在用患有急性呼吸道感染的感染患者的呼吸道材料接种后，在添加了葡聚糖硫酸酯钠的Caco-2培养物中证明了HBoV1的复制。展示了一个快速发展的病毒周期。然而，尽管到第1天时上清液和单层细胞中的HBoV1 DNA增加了4倍，证明该系统在病毒进入后允许病毒基因组复制，但未产生有感染性的子代颗粒。这些结果与仅限于单个生长周期的感染一致，这可能与HBoV1基因组的NS1和VP1/VP2区域中的突变有关。进一步的研究将有助于充分阐明这些观察结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/526567d3424d/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/bd587f9e287e/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/428a0eff5a0c/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/95b012bfe07d/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/d3156ab76568/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/2cbfaca65dc5/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/8b96935b3ced/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/526567d3424d/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/bd587f9e287e/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/428a0eff5a0c/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/95b012bfe07d/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/d3156ab76568/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/2cbfaca65dc5/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/8b96935b3ced/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e4d/7172243/526567d3424d/gr7_lrg.jpg

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