Chorpenning F W, Cooper H R, Rosen S
Infect Immun. 1975 Sep;12(3):586-91. doi: 10.1128/iai.12.3.586-591.1975.
Antisera to the whole cells of Streptococcus mutans cross-reacted with antigen extracts from four other gram-positive species, as well as with those of three other oral streptococci. Similarly, antisera to these bacteria cross-reacted with extracts from S. mutans and with those from each other. Using a purified phenol extract of the walls of S. mutans, which was identified by chemical, immunochemical, and enzymatic analyses as glycerol teichoic acid, the cross-reactions were shown to be specific for a determinant of the teichoic acid backbone. Results were confirmed in immunodiffusion tests where clear bands of identify were shown. These observations point out the need for caution in sereological research empolying extracts of gram-positive bacteria and may be of interest in investigations of periodontal disease.
变形链球菌全细胞抗血清与其他四种革兰氏阳性菌的抗原提取物以及其他三种口腔链球菌的抗原提取物发生交叉反应。同样，这些细菌的抗血清与变形链球菌的提取物以及彼此的提取物发生交叉反应。使用经化学、免疫化学和酶分析鉴定为甘油磷壁酸的变形链球菌细胞壁纯化酚提取物，表明交叉反应对磷壁酸主链的一个决定簇具有特异性。免疫扩散试验证实了结果，其中显示出清晰的同一性条带。这些观察结果指出，在使用革兰氏阳性菌提取物进行血清学研究时需要谨慎，并且可能对牙周病的研究有意义。
