Stelmasiak Z, Kozioł-Montewka M, Dobosz B, Rejdak K, Bartosik-Psujek H, Mitosek-Szewczyk K, Belniak-Legieć E
Department of Neurology, Medical University, ul. Lubartowska 85, 20-123 Lublin, Poland.
Med Sci Monit. 2000 Nov-Dec;6(6):1104-8.
The aim of the investigation was to determine pleiotropic cytokine IL-6 concentrations in serum and CSF of MS patients, with regard to clinical circumstances.
The study covered 27 MS patients: 23-51 years old; in the 2-7 clinical stage according to Kurtzke's Scale; mean 3.6 +/- 1.8; disease duration time ranged between 2-22 years. Serum and CSF interleukin-6 were determined by the use of commercial ELISA kits.
Increase of Il-6 in serum of MS patients was found (12.1 +/- 1.8 pg/ml) in comparison to the control group (6.6 +/- 4.5 pg/ml) (p = 0.03). Concentration of IL-6 in CSF of MS patients was 13.4 +/- 1.77 pg/ml and in the majority of patients it was higher than in the serum. The highest mean of IL-6 concentrations in serum and CSF have been found in patients at the longer disease duration time, although there were not statistically significant. We have found some degree of dependence between serum IL-6 concentration and the level of disability in Kurtzke's scale.
IL-6 participation in the basic MS immune processes is confirmed by its elevated concentration in serum and CSF, its relation to disease severity with the more expressed rise in cerebrospinal fluid.
本研究旨在根据临床情况测定多发性硬化症（MS）患者血清和脑脊液中多效细胞因子白细胞介素-6（IL-6）的浓度。
该研究涵盖27例MS患者，年龄在23至51岁之间；根据库尔茨克量表处于临床2至7期，平均为3.6±1.8；病程在2至22年之间。血清和脑脊液中的白细胞介素-6通过使用商用酶联免疫吸附测定（ELISA）试剂盒进行测定。
与对照组（6.6±4.5 pg/ml）相比，发现MS患者血清中IL-6升高（12.1±1.8 pg/ml）（p = 0.03）。MS患者脑脊液中IL-6浓度为13.4±1.77 pg/ml，且大多数患者脑脊液中的浓度高于血清中的浓度。病程较长的患者血清和脑脊液中IL-6浓度的平均值最高，尽管无统计学意义。我们发现血清IL-6浓度与库尔茨克量表中的残疾程度之间存在一定程度的相关性。
血清和脑脊液中IL-6浓度升高，以及其与疾病严重程度的关系（脑脊液中升高更为明显）证实了IL-6参与MS的基本免疫过程。
