Butler K, Benghuzzi H, Puckett A
Department of Health Science, School of Health Related Professions, University of Mississippi Medical Center, Jackson, USA.
Pathol Res Pract. 2001;197(1):29-39. doi: 10.1078/0344-0338-00005.
It is well documented that several ceramic materials are highly compatible and non-immunogenic with host tissues. Recent studies have demonstrated the need for further investigation of these devices in vivo to further elucidate the possible mechanisms involved in biocompatibility. The purpose of this investigation was to study the morphological characteristics of the fibrous tissue capsule resulting from the implantation of aluminum calcium phosphate (ALCAP) and hydroxyapetite (HA) bioceramics. Implants of ALCAP and HA were implanted into 10 adult male rats subcutaneously (S/C) and intraperitoneally (I/P). At 90 days post-implantation, the animals were euthanized, and the ceramic devices, the fibrous tissue, and vital organs were harvested. Evaluation of routine stained sections (5 microm, hematoxylin & eosin) of the fibrous tissue capsule surrounding the HA and ALCAP ceramics revealed the following: 1) all the ceramic devices had fibrous connective tissue capsules of slightly varying degrees of thickness at the time of sacrifice, depending on the site of implantation and type of material, and 2) there were statistically significant differences (p < 0.05) in the numbers and types of cellular components with respect to implantation site. The number of macrophages, neutrophils, fibroblasts, degree of vascularity, and thickness of the fibrous tissue matrix was found to be statistically different between the S/C implanted ceramic groups. The number of macrophages, neutrophils, fibroblasts, and collagen content comparing the fibrous tissue surrounding the ALCAP and HA ceramics (I/P), was found to be statisically different.
有充分的文献记载，几种陶瓷材料与宿主组织具有高度的相容性且无免疫原性。最近的研究表明，需要对这些装置进行进一步的体内研究，以进一步阐明生物相容性可能涉及的机制。本研究的目的是研究植入磷酸铝钙（ALCAP）和羟基磷灰石（HA）生物陶瓷后形成的纤维组织囊的形态特征。将ALCAP和HA植入物皮下（S/C）和腹腔内（I/P）植入10只成年雄性大鼠体内。植入后90天，对动物实施安乐死，并取出陶瓷装置、纤维组织和重要器官。对围绕HA和ALCAP陶瓷的纤维组织囊的常规染色切片（5微米，苏木精和伊红）进行评估，结果如下：1）在处死时，所有陶瓷装置都有厚度略有不同的纤维结缔组织囊，这取决于植入部位和材料类型；2）关于植入部位，细胞成分的数量和类型存在统计学上的显著差异（p<0.05）。皮下植入陶瓷组之间，巨噬细胞、中性粒细胞、成纤维细胞的数量、血管化程度和纤维组织基质的厚度在统计学上存在差异。比较围绕ALCAP和HA陶瓷（腹腔内植入）的纤维组织，发现巨噬细胞、中性粒细胞、成纤维细胞的数量和胶原含量在统计学上存在差异。
