Butler K, Benghuzzi H, Bajpai P, Puckett A, Tucci M, Cason Z, England B
University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1997;33:233-9.
Fibrous tissue formation is often used as a screening method to determine the biocompatibility of orthopaedic and dental implanted material. In this investigation, porous implants of tricalcium phosphate-lysine delivery devices were implanted intraperitoneally (IP) using adult male rats as a model. The fibrous tissue surrounding the implant was studied histologically to determine the infiltration of inflammatory cells and other healing components. Fibrous capsular tissues were carefully dissected away from the capsule noting the tissue closest to the implanted material. Evaluation of the sections (5um, H&E) collected from various areas of the implants (n = 5 per group) revealed that: (1) The use of TCPL implants resulted in the formation of three distinct cell layers of fibrous tissue. (2) The fibrous tissue thickness was found to be directly proportional to the duration of the implant. (3) The infiltration of macrophages, polymorphonuclear leukocytes, and fibroblasts were present in all phases studied. (4) Ordered collagen was observed only at the end of the 6, 9, and 12 month phases. (5) Remarkable progression of vascularity was present in the second and third layers of the fibrous tissue. In conclusion, these observations confirm our previous finding using different bioceramic material.
纤维组织形成常被用作一种筛选方法，以确定骨科和牙科植入材料的生物相容性。在本研究中，以成年雄性大鼠为模型，将磷酸三钙-赖氨酸递送装置的多孔植入物腹腔内（IP）植入。对植入物周围的纤维组织进行组织学研究，以确定炎性细胞和其他愈合成分的浸润情况。小心地从包膜上分离出纤维包膜组织，并记录最接近植入材料的组织。对从植入物各个区域收集的切片（5微米，苏木精-伊红染色）进行评估（每组n = 5），结果显示：（1）使用TCPL植入物导致形成了三层不同的纤维组织细胞层。（2）发现纤维组织厚度与植入持续时间成正比。（3）在所研究的所有阶段均存在巨噬细胞、多形核白细胞和成纤维细胞的浸润。（4）仅在6、9和12个月阶段结束时观察到有序的胶原蛋白。（5）纤维组织的第二层和第三层出现了明显的血管生成进展。总之，这些观察结果证实了我们之前使用不同生物陶瓷材料的发现。
