Butler K, Benghuzzi H, Tucci S
University of Mississippi Medical Center, School of Health Related Professions, Department of Health Science, 2500 North State Street, Jackson, Mississippi 39216, USA.
Biomed Sci Instrum. 2001;37:19-24.
It is well demonstrated in the literature that polyethylene materials used in orthopedic applications are highly compatible and non-immunogenic with host tissues. However, recent studies in our laboratory have demonstrated the need for further study of these devices in vivo to further elucidate the mechanisms involved in biocompatibility. The purpose of this investigation was to study the tissue-implant response resulting from the implantation of ultra high molecular weight polyethylene (PE) coated with poly-L-lysine at 90 days post-implantation. In this investigation, implants of PE coated with poly-L-lysine and saline were implanted into 8 adult male rats subcutaneously (S/C) and intraperitoneally (I/P). At 90 days post-implantation, the animals were euthanized; and the PE devices, the fibrous tissue, and vital organs were harvested. Evaluation of routinely stained sections (5 microns, Hematoxylin & Eosin) of the fibrous tissue capsule surrounding the PE implants revealed the following: 1) all the devices had fibrous connective tissue capsules of slightly varying degrees of thickness surrounding them present at the time of sacrifice, 2) the thickness of the fibrous tissue capsule was dependent on the site of implantation and type of coating, 3) the thickness of the fibrous tissue capsules around the subcutaneous devices differed markedly than those retrieved from the peritoneal cavity, and 4) there were statistically significant differences (T-test, p < 0.05) in the numbers and types of cellular components with respect to implantation site and type of coating.
文献充分证明，骨科应用中使用的聚乙烯材料与宿主组织具有高度兼容性且无免疫原性。然而，我们实验室最近的研究表明，需要对这些装置进行进一步的体内研究，以进一步阐明生物相容性所涉及的机制。本研究的目的是研究植入聚-L-赖氨酸包被的超高分子量聚乙烯（PE）90天后的组织-植入物反应。在本研究中，将聚-L-赖氨酸包被的PE植入物和生理盐水植入8只成年雄性大鼠的皮下（S/C）和腹腔内（I/P）。植入90天后，对动物实施安乐死；然后取出PE装置、纤维组织和重要器官。对围绕PE植入物的纤维组织囊的常规染色切片（5微米，苏木精和伊红染色）进行评估，结果如下：1）在处死时，所有装置周围均有厚度略有不同的纤维结缔组织囊；2）纤维组织囊的厚度取决于植入部位和包被类型；3）皮下装置周围的纤维组织囊厚度与从腹腔取出的装置明显不同；4）关于植入部位和包被类型，细胞成分的数量和类型存在统计学显著差异（T检验，p<0.05）。
