Fukui N, Nakajima K, Tashiro T, Oda H, Nakamura K
Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Japan.
Clin Orthop Relat Res. 2001 Feb(383):250-8. doi: 10.1097/00003086-200102000-00029.
Adhesion is a serious complication after trauma or surgery. Because adhesion formation is essentially a fibrogenetic process, a series of growth factors are assumed to be involved in its development. If this is true, it may be possible that inhibition of the growth factor activity suppresses adhesion formation. The current study was conducted to verify this hypothesis on fibroblast growth factor-2 using an intraarticular adhesion model in the rabbit knee. Forty Japanese White rabbits were used. They were divided randomly into five groups of eight animals, and in three of them, activity of endogenous fibroblast growth factor-2 was suppressed locally by a neutralizing antibody. The remaining two groups served as controls, and formation of adhesions was evaluated 4 weeks after surgery. The results showed that the administration of the antibody reduced the extent of adhesions macroscopically, whereas histologic observation and collagen content measurement suggested the adhesion tissue was not affected significantly. Corresponding to the macroscopic findings, contraction of the knee was improved in the antibody groups. The findings showed that suppression of fibroblast growth factor-2 activity reduces adhesions. It is expected that control of the cytokine activity may become a novel method for reducing adhesions.
粘连是创伤或手术后的一种严重并发症。由于粘连形成本质上是一个纤维生成过程，一系列生长因子被认为参与其发展。如果真是这样，抑制生长因子活性可能会抑制粘连形成。本研究旨在利用兔膝关节内粘连模型验证关于成纤维细胞生长因子-2的这一假设。使用了40只日本白兔。它们被随机分为五组，每组8只动物，其中三组通过中和抗体局部抑制内源性成纤维细胞生长因子-2的活性。其余两组作为对照，术后4周评估粘连形成情况。结果显示，抗体给药在宏观上减少了粘连程度，而组织学观察和胶原含量测量表明粘连组织未受到显著影响。与宏观结果一致，抗体组膝关节的挛缩得到改善。这些结果表明，抑制成纤维细胞生长因子-2的活性可减少粘连。预计控制细胞因子活性可能成为减少粘连的一种新方法。
