Trkuljić M, Ostojić G, Taseski J
Vojnosanit Pregl. 2000 Sep-Oct;57(5):43-7.
Results of indirect antiglobulin test (IAT) adding polyethylene glycol (PEG) were compared with conventional IAT performed using bovine serume albumin (BSA) with the aim of prenatal protection of Rh(D) negative pregnant women. Investigation enrolled 986 samples of pregnant women sera and confirmed that the use of PEG-IAT increased the degree of detection of clinically significant antierythrocyte antibodies. Above all, form the Rhesus blood groups system (using exclusively PEG-IAT) was detected by one antibody of anti-D, anti-C, anti-e and two anti-E), while by using BSA, anti-e was not detected at all. Besides, the need for additional serologic techniques has been reduced (treating of erythrocytes by enzymes) and the work of laboratories for prenatal protection has been alleviated, and at the same time the quality of analyses was not diminished, which gave the preference to PEG-IAT compared to BSA-IAT in prenatal testing.
为了对Rh(D)阴性孕妇进行产前保护，将添加聚乙二醇（PEG）的间接抗球蛋白试验（IAT）结果与使用牛血清白蛋白（BSA）进行的传统IAT结果进行了比较。研究纳入了986例孕妇血清样本，证实使用PEG-IAT提高了临床显著抗红细胞抗体的检测程度。最重要的是，通过使用PEG-IAT（仅使用PEG-IAT）从恒河猴血型系统中检测到了抗-D、抗-C、抗-e抗体各一种以及两种抗-E抗体，而使用BSA时根本未检测到抗-e抗体。此外，减少了对额外血清学技术（用酶处理红细胞）的需求，减轻了产前保护实验室的工作，同时分析质量并未降低，这使得在产前检测中PEG-IAT比BSA-IAT更具优势。
