KAT1对气孔开放并非必不可少。
KAT1 is not essential for stomatal opening.
作者信息
Szyroki A, Ivashikina N, Dietrich P, Roelfsema M R, Ache P, Reintanz B, Deeken R, Godde M, Felle H, Steinmeyer R, Palme K, Hedrich R
机构信息
Max-Delbrück-Laboratorium, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.
出版信息
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2917-21. doi: 10.1073/pnas.051616698. Epub 2001 Feb 20.
Abstract
It is generally accepted that K(+) uptake into guard cells via inward-rectifying K(+) channels is required for stomatal opening. To test whether the guard cell K(+) channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1En-1, was isolated from an En-1 mutagenized Arabidopsis thaliana population. Stomatal action and K(+) uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the K(+) channels AKT1, AKT2/3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inward-rectifying K(+) currents across the plasma membrane of both wild-type and KAT1En-1 plants. This study demonstrates that multiple K(+) channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.
摘要
普遍认为，气孔开放需要通过内向整流钾通道将钾离子吸收到保卫细胞中。为了测试保卫细胞钾通道KAT1对气孔开放是否必不可少，从经En-1诱变的拟南芥群体中分离出了一个敲除突变体KAT1En-1。然而，KAT1缺陷型植株的气孔活动和钾离子吸收并未受到损害。对分离出的保卫细胞原生质体进行的逆转录PCR实验表明，除了KAT1之外，钾通道AKT1、AKT2/3、AtKC1和KAT2在这种细胞类型中也有表达。在刺入式测量中，完整的保卫细胞在野生型和KAT1En-1植株的质膜上均表现出内向整流钾电流。这项研究表明，保卫细胞中存在多种钾通道转录本，并且KAT1对气孔活动并非必不可少。
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